1887

Abstract

The genes encode an -acyl homoserine lactone (AHL)-dependent quorum-sensing system consisting of an AHL synthase that directs the synthesis of -octanoyl--homoserine lactone (ohl) and -hexanoyl--homoserine lactone and a transcriptional regulator. The virulence of mutants was examined in two animal models. Rats were infected with agar beads containing K56-2, K56-I2 ( : : Tp) or K56-R2 ( : : Tn-OT182). At 10 days post-infection, the extent of lung histopathological changes was significantly lower in lungs infected with K56-I2 or K56-R2 compared to the parent strain. Intranasal infections were performed in mice and their wild-type siblings. K56-2 was more virulent in both groups of mice. K56-I2 was the least virulent strain and was not invasive in the mice. OHL was readily detected in lung homogenates from mice infected with K56-2 but was only detected at levels slightly above background in a few mice infected with K56-I2. Lung homogenates from mice infected with K56-2 had significantly higher levels of the inflammatory mediators murine macrophage inflammatory protein-2, /, interleukin-1 and interleukin-6 than those from K56-I2-infected animals. These studies indicate that a functional CepIR quorum-sensing system contributes to the severity of infections. A zinc metalloprotease gene () was shown to be regulated by CepR and may be one of the factors that accounts for the difference in virulence between the mutant and the parent strain.

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2003-12-01
2020-09-28
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