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Volume 149, Issue 10

Identification and characterization of a novel autolysin (Aae) with adhesive properties from Free

Abstract

biofilm formation on polymer surfaces is considered a major pathogenicity factor in foreign-body-associated infections. Previously, the 148 kDa autolysin AtlE from . , which is involved in the initial attachment of the cells to polymer surfaces and also binds to the extracellular matrix protein vitronectin, was characterized. Here, the characterization of a novel autolysin/adhesin (Aae) in . s is described. Aae was identified as a 35 kDa surface-associated protein that has bacteriolytic activity and binds vitronectin. Its N-terminal amino acid sequence was determined and the respective gene, , was cloned. DNA-sequence analysis revealed that encodes a deduced protein of 324 amino acids with a predicted molecular mass of 35 kDa. Aae contains three repetitive sequences in its N-terminal portion. These repeats comprise features of a putative peptidoglycan binding domain (LysM domain) found in a number of enzymes involved in cell-wall metabolism and also in some adhesins. Expression of by and subsequent analysis revealed that Aae possesses bacteriolytic activity and adhesive properties. The interaction of Aae with fibrinogen, fibronectin and vitronectin was found to be dose-dependent and saturable and to occur with high affinity, by using the real-time Biomolecular Interaction Analysis (BIA). Aae binds to the A- and B-chains of fibrinogen and to the 29 kDa N-terminal fragment of fibronectin. In conclusion, Aae is a surface-associated protein with bacteriolytic and adhesive properties representing a new member of the staphylococcal autolysin/adhesins potentially involved in colonization.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Aae, autolysin/adhesin from S. epidermidis , BIA, Biomolecular Interaction Analysis , Fg, fibrinogen , Fn, fibronectin , His6, hexahistidine and Vn, vitronectin
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2003-10-01
2024-04-18
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Identification and characterization of a novel autolysin (Aae) with adhesive properties from Staphylococcus epidermidis
Microbiology 149, 2769 (2003); https://doi.org/10.1099/mic.0.26527-0
/content/journal/micro/10.1099/mic.0.26527-0
/content/journal/micro/10.1099/mic.0.26527-0
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