1887

Abstract

The melanogenic marine bacterium synthesizes R-bodies as revealed by transmission electron microscopy. These structures were previously described in some obligate symbionts of paramecia and some free-living bacteria, none of which was isolated from sea water. In other micro-organisms, the synthesis of R-bodies has been related to extrachromosomal elements. Accordingly, induction by mitomycin C or UV radiation resulted in the production of defective phages resembling bacteriocins, indicating that it is a lysogenic bacterium. Two mitomycin-C-resistant strains defective in prophage replication have been isolated. These mutants, and the previously obtained strains ngC1, T102 and T103, the latter mutated in the gene encoding a sensor histidine kinase, are affected not only in phage replication but also in polyphenol oxidase activities and melanin synthesis, suggesting a relationship between the control of all these processes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.26524-0
2003-09-01
2020-08-07
Loading full text...

Full text loading...

/deliver/fulltext/micro/149/9/mic1492679.html?itemId=/content/journal/micro/10.1099/mic.0.26524-0&mimeType=html&fmt=ahah

References

  1. Appleby J. L., Parkinson J. S., Bourret R. B.. 1996; Signal transduction via the multi-step phosphorelay: not necessarily a road less traveled. Cell86:845–848
    [Google Scholar]
  2. Beier C. L., Horn M., Michel R., Schweikert M., Görtz H.-D., Wagner M.. 2002; The genus Caedibacter comprises endosymbionts of Paramecium spp. related to the Rickettsiales ( Alphaproteobacteria ) and to Francisella tularensis ( Gammaproteobacteria ). Appl Environ Microbiol68:6043–6050
    [Google Scholar]
  3. Cochran P. K., Paul J. H.. 1998; Seasonal abundance of lysogenic bacteria in a subtropical estuary. Appl Environ Microbiol64:2308–2312
    [Google Scholar]
  4. Espuny M. J., Andrés C., Mercade M. E., Robert M., Manresa M. A., Guinea J.. 1991; R-bodies in Pseudomonas aeruginosa strain 44T1. Antonie van Leeuwenhoek60:83–86
    [Google Scholar]
  5. Favinger J., Stadtwald R., Gest H.. 1989; Rhodospirillum centenum , sp. nov. a thermotolerant cyst-forming anoxygenic photosynthetic bacterium. Antonie van Leeuwenhoek55:291–296
    [Google Scholar]
  6. Fusté M. C., Simon-Pujol M. D., Marques A. M., Guinea J., Congregrado F.. 1986; Isolation of a new free-living bacterium containing R-bodies. J Gen Microbiol132:2801–2805
    [Google Scholar]
  7. Hearing V., Tsukamoto K.. 1991; Enzymatic control of pigmentation in mammals. FASEB J5:2902–2909
    [Google Scholar]
  8. Heruth D. P., Pond F. R., Dilts J. A., Quackenbush R. L.. 1994; Characterization of genetic determinants for R body synthesis and assembly in Caedibacter taeniospiralis 47 and 116. J Bacteriol176:3559–3567
    [Google Scholar]
  9. Hu F.. 1981; Melanocyte cytology in normal skin, melanocyte nevi and malignant melanomas. In Pathology of Malignant Melanoma pp1–21 Edited by Ackerman A. B.. New York: Masson Publishing;
  10. Jiang S. C., Paul J. H.. 1998; Significance of lysogeny in the marine environment: studies with isolates and a model of lysogenic phage production. Microb Ecol35:235–243
    [Google Scholar]
  11. Kageyama M.. 1975; Bacteriocins and bacteriophages in Pseudomonas aeruginosa . In Microbial Drug Resistance pp291–305 Edited by Mitsuhashi S., Hashimoto H. Tokyo: University of Tokyo Press;
  12. Kanabrocki J. A., Quackenbush R. L., Pond F. R.. 1986; Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies. J Bacteriol168:40–48
    [Google Scholar]
  13. Lalucat J., Meyer O., Mayer F., Pares R., Schlegel H. G.. 1979; R-bodies in newly isolated free-living hydrogen-oxidizing bacteria. Arch Microbiol121:9–15
    [Google Scholar]
  14. Lee F. K. N., Dudas K. C., Hanson J. A., Nelson M. B., LoVerde P. T., Apicella M. A.. 1999; The R-type pyocin of Pseudomonas aeruginosa C is a bacteriophage tail-like particle that contains single-stranded DNA. Infect Immun67:717–725
    [Google Scholar]
  15. López-Serrano D., Sanchez-Amat A., Solano F.. 2002; Cloning and molecular characterization of a SDS-activated tyrosinase from Marinomonas mediterranea . Pigment Cell Res15:104–111
    [Google Scholar]
  16. Lucas-Elío P., Solano F., Sanchez-Amat A.. 2002; Regulation of polyphenol oxidase activities and melanin synthesis in Marinomonas mediterranea : identification of ppoS , a gene encoding a sensor histidine kinase. Microbiology148:2457–2466
    [Google Scholar]
  17. Petroni G., Spring S., Schleifer K. H., Verni F., Rosati G.. 2000; Defensive extrusive ectosymbionts of Euplotidium ( Ciliophora ) that contain microtubule-like structures are bacteria related to Verrucomicrobia . Proc Natl Acad Sci U S A97:1813–1817
    [Google Scholar]
  18. Pond F. R., Gibson I., Lalucat J., Quackenbush R. L.. 1989; R-body-producing bacteria. Microb Rev53:25–67
    [Google Scholar]
  19. Preer J. R., Preer L. B., Jurand A.. 1974; Kappa and other endosymbionts in Paramecium aurelia . Bacteriol Rev38:113–163
    [Google Scholar]
  20. Riley P. A.. 1992; Materia melanica: further dark thoughts. Pigment Cell Res5:101–106
    [Google Scholar]
  21. Sanchez-Amat A., Solano F.. 1997; A pluripotent polyphenol oxidase from the melanogenic marine Alteromonas sp. shares catalytic capabilities of tyrosinases and laccases. Biochem Biophys Res Commun240:787–792
    [Google Scholar]
  22. Sanchez-Amat A., Lucas-Elio P., Fernández E., Garcia-Borrón J. C., Solano F.. 2001; Molecular cloning and functional characterization of a unique multipotent polyphenol oxidase from Marinomonas mediterranea . Biochim Biophys Acta 1547;104–116
    [Google Scholar]
  23. Shinomiya T., Ina S.. 1989; Genetic comparison of bacteriophage PS17 and Pseudomonas aeruginosa R-type pyocin. J Bacteriol171:2287–2292
    [Google Scholar]
  24. Solano F., Sanchez-Amat A.. 1999; Studies on the phylogenetic relationships of melanogenic marine bacteria: proposal of Marinomonas mediterranea sp. nov. Int J Syst Bacteriol49:1241–1246
    [Google Scholar]
  25. Solano F., García E., Pérez de Egea E., Sanchez-Amat A.. 1997; Isolation and characterization of strain MMB-1 (CECT 4803), a novel melanogenic marine bacterium. Appl Environ Microbiol63:3499–3506
    [Google Scholar]
  26. Solano F., Lucas-Elío P., Fernández E., Sanchez-Amat A.. 2000; Marinomonas mediterranea MMB-1 transposon mutagenesis: isolation of a multipotent polyphenol oxidase mutant. J Bacteriol182:3754–3760
    [Google Scholar]
  27. Strauch E., Kaspar H., Schaudinn C., Dersch P., Madela K., Gewinner C., Hertwig S., Wecke J., Appel B.. 2001; Characterization of enterocoliticin, a phage tail-like bacteriocin, and its effect on pathogenic Yersinia enterocolitica strains. Appl Environ Microbiol67:5634–5642
    [Google Scholar]
  28. Thaler J. O., Baghdiguian S., Boemare N.. 1995; Purification and characterization of xenorhabdicin, a phage tail-like bacteriocin, from the lisogenic strain F1 of Xenorhabdus nematophilus . Appl Environ Microbiol61:2049–2052
    [Google Scholar]
  29. Wells B., Horne R. W.. 1983; The ultrastructure of Pseudomonas avenae . II. Intracellular refractile (R-body) structure. Micron Microsc Acta14:329–344
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.26524-0
Loading
/content/journal/micro/10.1099/mic.0.26524-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error