1887

Abstract

Poly(ethylene oxide) (PEO)-brushes are generally recognized as protein-repellent surfaces, and although a role in discouraging microbial adhesion has been established for some strains and species, no study exists on the effects of PEO-brushes on a large variety of bacterial and yeast strains. In this paper, a PEO-brush has been covalently attached to glass and silica by reaction in a polymer melt. Subsequently, the presence of a PEO-brush was demonstrated using contact angle measurements, X-ray photoelectron spectroscopy and ellipsometry. For five bacterial (, , , and ) and two yeast strains ( and ), adhesion to PEO-brushes was compared with adhesion to bare glass in a parallel plate flow chamber. The initial deposition rates of , and to glass were relatively high, between 2400 and 2600 cm s, while and deposited much more slowly. The initial deposition rates of the yeasts to glass were 144 and 444 cm s for GB 1/2 and GB 9/9, respectively. Coating of the glass surface with a PEO-brush yielded more than 98 % reduction in bacterial adhesion, although for the more hydrophobic a smaller reduction was observed. For both yeast species adhesion suppression was less effective than for the bacteria and here too the more hydrophobic showed less reduction than the more hydrophilic . The PEO-brush had a thickness of 22 nm in water, as inferred from ellipsometry. Assuming that on bare glass the adhered micro-organisms are positioned only a few nanometers away from the surface and that the brush keeps them at a distance of 22 nm, it is calculated that the brush yields a sevenfold attenuation of the Lifshitz–Van der Waals attraction to the surface between the micro-organisms and the surface. Decreased Lifshitz–van der Waals attraction may be responsible for the suppression of the microbial adhesion observed.

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2003-11-01
2019-10-14
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References

  1. Andrade, J. D., Smith, L. M. & Gregonis, D. E. ( 1985; ). The contact angle and interface energetics. In Surface and Interfacial Aspects of Biomedical Polymers, pp. 249–292. Edited by J. D. Andrade. New York: Plenum.
  2. Bos, R., Van der Mei, H. C. & Busscher, H. J. ( 1999; ). Physico-chemistry of initial microbial adhesive interactions – its mechanisms and methods for study. FEMS Microbiol Rev 23, 179–230.
    [Google Scholar]
  3. Bridgett, M. J., Davies, M. C. & Denyer, S. P. ( 1992; ). Control of staphylococcal adhesion to polystyrene surfaces by polymer surface modification with surfactants. Biomaterials 13, 411–416.[CrossRef]
    [Google Scholar]
  4. Busscher, H. J. & Van der Mei, H. C. ( 1995; ). Use of flow chamber devices and image analysis methods to study microbial adhesion. In Adhesion of Microbial Pathogens, pp. 455–476. Edited by R. J. Doyle & I. Ofek. San Diego: Academic Press.
  5. Busscher, H. J. & Weerkamp, A. H. ( 1987; ). Specific and non-specific interactions in bacterial adhesion to solid substrata. FEMS Microbiol Rev 46, 165–173.[CrossRef]
    [Google Scholar]
  6. Busscher, H. J., Geertsema-Doornbusch, G. I. & Van der Mei, H. C. ( 1997; ). Adhesion to silicone rubber of yeasts and bacteria isolated from voice prostheses: influence of salivary conditioning films. J Biomed Mater Res 34, 201–209.[CrossRef]
    [Google Scholar]
  7. Currie, E. P. K., Norde, W. & Cohen Stuart, M. A. ( 2003; ). Tethered polymer chains: surface chemistry and their impact on colloidal and surface properties. Adv Colloid Interface Sci 100–102, 205–265.
    [Google Scholar]
  8. Efremova, N. V., Sheth, S. R. & Leckband, D. E. ( 2001; ). Protein-induced changes in poly(ethylene glycol) brushes: molecular weight and temperature dependence. Langmuir 17, 7628–7636.[CrossRef]
    [Google Scholar]
  9. Everaert, E. P. J. M., Van de Belt-Gritter, B., Van der Mei, H. C., Busscher, H. J., Verkerke, G. J., Dijk, F., Mahieu, H. F. & Reitsma, A. ( 1998; ). In vitro and in vivo microbial adhesion and growth on argon plasma-treated silicone rubber voice prostheses. J Mater Sci Mater Med 9, 147–157.[CrossRef]
    [Google Scholar]
  10. Furness, E. L., Ross, A., Davis, T. P. & King, G. C. ( 1998; ). A hydrophobic interaction site for lysozyme binding to polyethylene glycol and model contact lens polymers. Biomaterials 19, 1361–1369.[CrossRef]
    [Google Scholar]
  11. Gòmez-Suárez, C., Busscher, H. J. & Van der Mei, H. C. ( 2001; ). Analysis of bacterial detachment from substratum surfaces by the passage of air–liquid interfaces. Appl Environ Microbiol 67, 2531–2537.[CrossRef]
    [Google Scholar]
  12. Gottenbos, B., Van der Mei, H. C. & Busscher, H. J. ( 1999; ). Models for studying initial adhesion and surface growth in biofilm formation on surfaces. Methods Enzymol 310, 523–534.
    [Google Scholar]
  13. Gottenbos, B., Grijpma, D. W., Van der Mei, H. C., Feijen, J. & Busscher, H. J. ( 2001; ). Antimicrobial effects of positively charged surfaces on adhering Gram-positive and Gram-negative bacteria. J Antimicrob Chemother 48, 7–13.[CrossRef]
    [Google Scholar]
  14. Gristina, A. G. ( 1987; ). Biomaterial-centered infection: microbial adhesion versus tissue integration. Science 237, 1588–1595.[CrossRef]
    [Google Scholar]
  15. Halperin, A. ( 1999; ). Polymer brushes that resist adsorption of model proteins: design parameters. Langmuir 15, 2525–2533.[CrossRef]
    [Google Scholar]
  16. Harder, P., Grunze, M., Dahint, R., Whitesides, G. M. & Laibinis, P. E. ( 1998; ). Molecular conformation in oligo(ethylene glycol)-terminated self-assembled monolayers on gold and silver surfaces determines their ability to resist protein adsorption. J Phys Chem B 102, 426–436.
    [Google Scholar]
  17. Harris, J. ( 1992; ). Poly(ethyleneglycol) Chemistry: Biotechnical and Biomedical Applications. New York: Plenum.
  18. Hermansson, M. ( 1999; ). The DLVO theory in microbial adhesion. Colloids Surf B 14, 105–119.[CrossRef]
    [Google Scholar]
  19. Ista, L. K., Fan, H., Baca, O. & Lopez, G. P. ( 1996; ). Attachment of bacteria to model solid surfaces: oligo(ethylene glycol) surfaces inhibit bacterial attachment. FEMS Microbiol Lett 142, 59–63.[CrossRef]
    [Google Scholar]
  20. Jeon, S. I., Lee, J. H., Andrade, J. D. & Gennes, P. G. ( 1991; ). Protein–surface interactions in the presence of polyethylene oxide. J Colloid Sci 142, 149–165.[CrossRef]
    [Google Scholar]
  21. Kasemo, B. & Lausmaa, J. ( 1988; ). Biomaterial and implant surfaces – on the role of cleanliness, contamination, and preparation procedures. J Biomed Mater Res Appl Biomater 22, 145–158.[CrossRef]
    [Google Scholar]
  22. Lee, S. W. & Laibinis, P. E. ( 1998; ). Protein-resistant coatings for glass and metal oxide surfaces derived from oligo(ethylene glycol)-terminated alkyltrichlorosilanes. Biomaterials 19, 1669–1675.[CrossRef]
    [Google Scholar]
  23. Maas, J. H., Cohen Stuart, M. A., Sieval, A. B., Zuilhof, H. & Sudholter, E. J. R. ( 2003; ). Preparation of polystyrene brushes by reaction of terminal vinyl groups on silicon and silica surfaces. Thin Solid Films 426, 135–139.[CrossRef]
    [Google Scholar]
  24. Mafu, A. A., Roy, D., Goulet, J. & Savoie, L. ( 1991; ). Characterization of physicochemical forces involved in adhesion of Listeria monocytogenes to surfaces. Appl Environ Microbiol 57, 1969–1973.
    [Google Scholar]
  25. Morra, M. ( 2000; ). On the molecular basis of fouling resistance. J Biomater Sci Polym Ed 11, 547–569.[CrossRef]
    [Google Scholar]
  26. Park, K. D., Kim, Y. S., Han, D. K., Kim, Y. H., Lee, E. H., Suh, H. & Choi, K. S. ( 1998; ). Bacterial adhesion on PEG modified polyurethane surfaces. Biomaterials 19, 851–859.[CrossRef]
    [Google Scholar]
  27. Razatos, A., Ong, Y. L., Boulay, F., Elbert, D. L., Hubbell, J. A., Sharma, M. M. & Georgiou, G. ( 2000; ). Force measurements between bacteria and poly(ethylene glycol)-coated surfaces. Langmuir 16, 9155–9158.[CrossRef]
    [Google Scholar]
  28. Rijnaarts, H. H. M., Norde, W., Bouwer, E. J., Lyklema, J. & Zehnder, A. J. B. ( 1995; ). Reversibility and mechanism of bacterial adhesion. Colloids Surf B 4, 5–22.[CrossRef]
    [Google Scholar]
  29. Rutter, P. R. ( 1980; ). The physical chemistry of the adhesion of bacteria and other cells. In Cell Adhesion and Motility, pp. 103–135. Edited by A. S. G. Curtis & J. D. Pitts. Cambridge: Cambridge University Press.
  30. Seah, M. P., Qui, J. H., Cumpson, P. J. & Castle, J. E. ( 1994; ). Simple method of depth profiling (stratifying) contamination layers, illustrated by studies on stainless-steel. Surf Interface Anal 21, 336–341.[CrossRef]
    [Google Scholar]
  31. Sheth, S. R., Efremova, N. & Leckband, D. E. ( 2000; ). Interactions of poly(ethylene oxide) brushes with chemically selective surfaces. J Phys Chem B 104, 7652–7662.[CrossRef]
    [Google Scholar]
  32. Tsibouklis, J., Stone, M., Thorpe, A. A., Graham, P., Peters, V., Heerlien, R., Smith, J. R., Green, K. L. & Nevell, T. G. ( 1999; ). Preventing bacterial adhesion onto surfaces: the low energy approach. Biomaterials 20, 1229–1235.[CrossRef]
    [Google Scholar]
  33. Van der Mei, H. C., Bos, R. & Busscher, H. J. ( 1998; ). A reference guide to microbial cell surface hydrophobicity based on contact angles. Colloids Surf B 11, 213–221.[CrossRef]
    [Google Scholar]
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