1887

Abstract

Hyphal tip growth has been previously reported as pulsatile, defined as regularly alternating fast and slow rates of extension. The growth of pollen tubes, and hyphae of and were analysed using high spatial and temporal resolution. By using long (100–500 s) records of growth rate, sampled every second, it was possible to apply rigorous statistical analysis of the time series. As previously demonstrated, pollen tubes can show pulsatile growth, detectable with this system. In contrast, hyphal growth rates do not show any evidence of pulsatile growth; instead, growth rates appear to fluctuate randomly. It is concluded that pulsatile growth is not a common feature of hyphal tip growth.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.26509-0
2003-11-01
2020-04-07
Loading full text...

Full text loading...

/deliver/fulltext/micro/149/11/mic1493111.html?itemId=/content/journal/micro/10.1099/mic.0.26509-0&mimeType=html&fmt=ahah

References

  1. Castle E. S.. 1940; Discontinuous growth of single plant cells measured at short intervals, and the theory of intussusception. J Cell Comp Physiol15:285–298
    [Google Scholar]
  2. Feijó J., Sainhas J., Holdaway-Clarke T., Cordeiro M., Kunkel J., Hepler P.. 2001; Cellular oscillations and the regulation of growth: the pollen tube paradigm. BioEssays23:86–94
    [Google Scholar]
  3. Heath I. B., Greenwood A. D.. 1970; The structure and formation of lomasomes. J Gen Microbiol62:129–137
    [Google Scholar]
  4. Holdaway-Clarke T., Feijó J., Hackett G., Kunkel J., Hepler P.. 1997; Pollen tube growth and the intracellular cytosolic calcium gradient oscillate in phase while extracellular calcium influx is delayed. Plant Cell9:1999–2010
    [Google Scholar]
  5. Jackson S.. 2001; Do hyphae pulse as they grow?. New Phytol151:556–560
    [Google Scholar]
  6. Johns S., Davis C., Money N.. 1999; Pulses in turgor pressure and water potential: resolving the mechanics of hyphal growth. Microbiol Res154:225–231
    [Google Scholar]
  7. Legendre L., Legendre P.. 1983; Numerical Ecology Amsterdam: Elsevier;
  8. López-Franco R., Bartnicki-Garcia S., Bracker C. E.. 1994; Pulsed growth of fungal hyphal tips. Proc Natl Acad Sci U S A91:12228–12232
    [Google Scholar]
  9. MacDonald D. K. C.. 1962; Noise and Fluctuations: an Introduction New York: Wiley;
  10. Messerli M., Robinson K. R.. 1997; Tip localized Ca2+ pulses are coincident with peak pulsatile growth rates in pollen tubes of Lillium longiflorum. J Cell Sci110:1269–1278
    [Google Scholar]
  11. Messerli M. A., Danuser G., Robinson K. R.. 1999; Pulsatile influxes of H+, K+ and Ca2+ lag growth pulses of Lilium longiflorum pollen tubes. J Cell Sci112:1497–1509
    [Google Scholar]
  12. Messerli M., Créton R., Jaffe L., Robinson K.. 2000; Periodic increases in elongation rate precede increases in cytosoloic Ca2+ during pollen tube growth. Dev Biol222:84–98
    [Google Scholar]
  13. Money N. P.. 1997; Wishful thinking of turgor revisited: the mechanics of fungal growth. Fungal Genet Biol21:173–187
    [Google Scholar]
  14. Money N.. 2001; The pulse of the machine-reevaluating tip-growth methodology. New Phytol151:553–555
    [Google Scholar]
  15. NIST/SEMATECH 2003; NIST/SEMATECH e-Handbook of Statistical Methods. January 2003
  16. Pierson E. S., Miller D. D., Callaham D. A., Aken J. V., Hackett G., Hepler P. K.. 1996; Tip-localized calcium entry fluctuates during pollen tube growth. Dev Biol174:160–173
    [Google Scholar]
  17. Shaw S., Dumais J., Long S.. 2000; Cell surface expansion in polarly growing root hairs of Medicago truncatula. Plant Physiol124:959–969
    [Google Scholar]
  18. Wilkinson L.. 1990; SYSTAT: the System for Statistics Evanston: SYSTAT;
  19. Wymer C., Bibikova T., Gilroy S.. 1997; Cytoplasmic free calcium distributions during the development of root hairs of Arabidopsis thaliana. Plant J12:427–439
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.26509-0
Loading
/content/journal/micro/10.1099/mic.0.26509-0
Loading

Data & Media loading...

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error