1887

Abstract

This study has revealed that a ferredoxin gene (per-) possesses a novel type of DNA curvature, which is formed by five phased A-tracts extending from upstream to downstream of the −35 region. The three A-tracts upstream of the promoter and the two within the promoter are located at the positions corresponding to A-tracts present in a phospholipase C gene () and a ferredoxin gene (pas-), respectively. DNA fragments of the per-, pas- and genes (nucleotide positions −69 to +1 relative to the transcription initiation site) were fused to a chloramphenicol acetyltransferase reporter gene on a plasmid, pPSV, and their promoter activities were examined by assaying the chloramphenicol acetyltransferase activity of each transformant. Comparison of the three constructs showed that the order of promoter activity is, in descending order, per-, pas- and . Deletion of the three upstream A-tracts of the per- gene drastically decreased the promoter activity, as demonstrated previously for the promoter. Substitution of the most downstream A-tract decreased the promoter activities of the per- and pas- genes. These results indicate that not only the phased A-tracts upstream of the promoter but also those within the promoter stimulate the promoter activity, and suggest that the high activity of the per- promoter is due to the combined effects of these two types of A-tracts.

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2003-11-01
2020-04-09
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