1887

Abstract

The full sequence of plasmid p250, isolated from strain HP250, has been obtained. The plasmid contains seven ORFs: a putative integrase, a putative replication protein () and five ORFs similar to those from the haemocin (bacteriocin) operon from . Of 19 other non-plasmid-containing strains screened (11 serovar reference strains and 8 field isolates), 17 strains produced haemocin and were resistant to killing by strain HP250. These strains, unlike strain HP250, have a chromosomally encoded haemocin operon. A number of other members of the family were tested for haemocin sensitivity. , and species A, all non-pathogenic bacteria found in the respiratory tract of chickens suffering from respiratory diseases, were sensitive to haemocin. However, amongst the pathogenic , 50 % of isolates and all five isolates of tested were sensitive to the haemocin. Given the prevalence of haemocin production in strains, it may play a role in aiding colonization by inhibiting other Gram-negative bacteria that are associated with the respiratory tract in chickens. The origin of replication from plasmid p250 has been used to generate an shuttle vector which may be useful in genetically manipulating .

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2003-11-01
2019-10-24
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