1887

Abstract

assimilates ammonium by the concerted action of glutamine synthetase and glutamate synthase. The expression of the operon encoding the latter enzyme is impaired in mutant strains. CcpA is a pleiotropic transcriptional regulator that is the key factor in the regulation of carbon metabolism. However, in addition to their defect in catabolite repression mutants are unable to grow on minimal media with glucose and ammonium as the single sources of carbon and nitrogen, respectively. In this work, the expression of the operon was analysed and its role in growth on minimal sugar/ammonium media was studied. Expression of requires induction by glucose or other glycolytically catabolized carbon sources. In mutants, cannot be induced by glucose due to the low activity of the phosphotransferase sugar transport system in these mutants. A mutation that allowed phosphotransferase system activity in a background simultaneously restored glucose induction of and growth on glucose/ammonium medium. Moreover, artificial induction of the operon in the mutant allowed the mutant strain to grow on minimal medium with glucose and ammonium. It may be concluded that expression of the operon depends on the accumulation of glycolytic intermediates which cannot occur in the mutant. The lack of induction is the bottleneck that prevents growth of the mutant on glucose/ammonium media. The control of expression of the operon by CcpA provides a major regulatory link between carbon and amino acid metabolism.

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2003-10-01
2020-02-25
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