1887

Abstract

A novel, fast-growing spirochaete was isolated from the hard tick (family , subfamily ) using Barbour–Stoenner–Kelly (BSK) II medium. Tick samples were taken during the summer of 2000 from the Istanbul area in northwestern Turkey. Sixty-seven of 153 adults (44 %) and 72 of 185 nymphs (39 %) were infected with the novel spirochaete, whereas none of the 20 larvae examined were infected. The optimal growth temperature of the spirochaete in BSK II medium was 34–37 °C, and it could grow at 39 °C. Doubling times at 34 and 37 °C were 5·3 and 5·1 h, respectively. Six pure cultures of the spirochaete were obtained and characterized by microscopic observation, sequence analysis of the flagellin gene (), SDS-PAGE and Western blotting. The spirochaete was morphologically similar to those of the genus and contained a 41 kDa protein reactive with mAb H9724 specific to the flagellin of a species. Polyclonal antibody raised to this spirochaete reacted with several antigen bands, whereas no bands were detected with , , and . The sequences of the six isolates showed high similarity, with sequence similarity values ranging from 99·2 to 100 %; however, the similarity of the isolates' sequences to those of the Lyme-disease-related and relapsing-fever-associated species was less than 90 %. These findings suggest that the unique spirochaete is a member of the genus , and differs from previously described species.

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2003-09-01
2024-11-13
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References

  1. Baltazard M. 1953; Sur le classement des spirochetes recurrentis . Bull Soc Pathol Exot 47:10–29
    [Google Scholar]
  2. Barbour A. G. 1984; Isolation and cultivation of Lyme disease spirochetes. Yale J Biol Med 57:521–555
    [Google Scholar]
  3. Barbour A. G., Hayes S. F. 1986; Biology of Borrelia species. Microbiol Rev 50:381–400
    [Google Scholar]
  4. Barbour A. G., Tessier S. L., Todd W. J. 1983; Lyme disease spirochetes and ixodid tick spirochetes share a common surface antigenic determinant defined by a monoclonal antibody. Infect Immun 41:795–804
    [Google Scholar]
  5. Barbour A. G., Hayes S. F., Heiland R. A., Schrumpf M. E., Tessier S. L. 1986; A Borrelia -specific monoclonal antibody binds to a flagellar epitope. Infect Immun 52:549–554
    [Google Scholar]
  6. Barbour A. G., Maupin G. O., Teltow G. J., Carter C. J., Piesman J. 1996; Identification of an uncultivable Borrelia species in the hard tick Amblyomma americanum : possible agent of a Lyme disease-like illness. J Infect Dis 173:403–409
    [Google Scholar]
  7. Cutler S. J., Akintunde C. O., Moss J., Fukunaga M., Kurtenbach K., Talbert A., Zhang H., Wright D. J., Warrell D. A. 1999; Successful in vitro cultivation of Borrelia duttonii and its comparison with Borrelia recurrentis . Int J Syst Bacteriol 49:1793–1799
    [Google Scholar]
  8. De Michelis S., Sewell H. S., Collares-Pereira M., Santos-Reis M., Schouls L. M., Benes V., Holmes E. C., Kurtenbach K. 2000; Genetic diversity of Borrelia burgdorferi sensu lato in ticks from mainland Portugal. J Clin Microbiol 38:2128–2133
    [Google Scholar]
  9. Fukunaga M., Takahashi Y., Tsuruta Y., Matsushita O., Ralph D., McClelland M., Nakao M. 1995; Genetic and phenotypic analysis of Borrelia miyamotoi sp. nov., isolated from the ixodid tick Ixodes persulcatus , the vector for Lyme disease in Japan. Int J Syst Bacteriol 45:804–810
    [Google Scholar]
  10. Güner E. S., Hashimoto N., Takada N., Kaneda K., Imai Y., Masuzawa T. 2003; First isolation and characterization of Borrelia burgdorferi sensu lato strains from Ixodes ricinus ticks in Turkey. J Med Microbiol 52:807–813
    [Google Scholar]
  11. Kelly R. T. 1984; Genus IV. Borrelia Swellengrebel 1907, 582AL . In Bergey's Manual of Systematic Bacteriology vol. 1 pp 57–62 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  12. Masuzawa T., Okada Y., Yanagihara Y., Sato N. 1991; Antigenic properties of Borrelia burgdorferi isolated from Ixodes ovatus and Ixodes persulcatus in Hokkaido, Japan. J Clin Microbiol 29:1568–1573
    [Google Scholar]
  13. Masuzawa T., Suzuki H., Kawabata H., Ishiguro F., Takada N., Yanagihara Y. 1996a; Characterization of Borrelia spp. isolated from the tick, Ixodes tanuki and small rodents in Japan. J Wildl Dis 32:565–571
    [Google Scholar]
  14. Masuzawa T., Komikado T., Iwaki A., Suzuki H., Kaneda K., Yanagihara Y. 1996b; Characterization of Borrelia sp. isolated from Ixodes tanuki , I. turdus , and I. columnae in Japan by restriction fragment length polymorphism of rrf (5S)– rrl (23S) intergenic spacer amplicons. FEMS Microbiol Lett 142:77–83
    [Google Scholar]
  15. Masuzawa T., Takada N., Kudeken M., Fukui T., Yano Y., Ishiguro F., Kawamura Y., Imai Y., Ezaki T. 2001; Borrelia sinica sp. nov., a Lyme disease-related Borrelia species isolated in China. Int J Syst Evol Microbiol 51:1817–1824
    [Google Scholar]
  16. Merdivenci A. 1969 Investigations on ticks of Turkey Professorship thesis Istanbul University Cerrahpasa Medical School Publishing (in Turkish;
  17. Miyamoto K., Masuzawa T. 2002 Ecology of Borrelia burgdorferi sensu lato in Japan and East Asia. In Lyme Borreliosis, Biology, Epidemiology and Control pp 201–222 Edited by Gray J. S., Kahl O., Lane R. S., Stanek G. Oxford: CABI Publishing;
    [Google Scholar]
  18. Ozsan K., Akyay N. 1954; Tick-borne relapsing fever in Turkey: presence of O. erraticue infected with spirochetes of crocidurae group at Turkey–Syria border. Turkish J Hyg Biotech 3:34–36 in Turkish
    [Google Scholar]
  19. Parola P., Raoult D. 2001; Ticks and tickborne bacterial diseases in humans: an emerging infectious threat. Clin Infect Dis 32:897–928
    [Google Scholar]
  20. Postic D., Assous M. V., Grimont P. A., Baranton G. 1994; Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)– rrl (23S) intergenic spacer amplicons. Int J Syst Bacteriol 44:743–752
    [Google Scholar]
  21. Ras N. M., Lascola B., Postic D., Cutler S. J., Rodhain F., Baranton G., Raoult D. 1996; Phylogenesis of relapsing fever Borrelia spp. Int J Syst Bacteriol 46:859–865
    [Google Scholar]
  22. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  23. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882
    [Google Scholar]
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