1887

Abstract

A constitutively active mutant of histidine kinase sensor EvgS was found to confer multi-drug resistance (MDR) to an -deficient , indicating the relationship between the two-component system EvgAS and the expression of the MDR system. The observed MDR also depended on an outer-membrane channel, TolC. Microarray and S1 mapping assays indicated that, in the presence of this constitutive mutant EvgS, the level of transcription increased for some MDR genes, including the drug efflux genes , , , and . Transcription of increased by the addition of phosphorylated EvgA. Transcription activation of by the activated EvgS was, however, dependent on both EvgAS and PhoPQ (Mg-responsive two-component system), in agreement with the presence of the binding site (PhoP box) for the regulator PhoP in the promoter region. Transcription of also appears to require an as-yet-unidentified additional transcriptional factor besides EvgA. Taken together we propose that the expression of the MDR system is under a complex regulatory network, including the phosphorylated EvgA serving as the master regulator.

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2003-10-01
2019-10-20
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