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Volume 149, Issue 10

() gene, involved in coat assembly and spore resistance Free

Abstract

In systematic screening four sporulation-specific genes, , , and , of unknown function were found in . These genes are located just upstream of the gene cluster oriented in the opposite direction. Northern blot analysis showed that was transcribed by the SigE RNA polymerase beginning 2 h ( ) after the onset of sporulation, and , and were transcribed by the SigK RNA polymerase beginning at of sporulation. The transcription of was dependent on SigK and GerE. The consensus sequences of the appropriate sigma factors were found upstream of each gene. There were putative GerE-binding sites upstream of . Insertional inactivation of the gene resulted in a reduction in resistance of the mutant spores to lysozyme and heat. Transmission electron microscopic examination of spores revealed a defect of sporulation at stage VI, resulting in loss of spore coats. These results suggest that YjcC is involved in assembly of spore coat proteins that have roles in lysozyme resistance. It is proposed that should be renamed as .

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
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2003-10-01
2024-04-24
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Bacillus subtilis spoVIF (yjcC) gene, involved in coat assembly and spore resistance
Microbiology 149, 3011 (2003); https://doi.org/10.1099/mic.0.26432-0
/content/journal/micro/10.1099/mic.0.26432-0
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