1887

Abstract

binds to human desquamated nasal epithelial cells, a phenomenon likely to be important in nasal colonization. ClfB was identified previously as one staphylococcal adhesin that promoted binding to nasal epithelia. In this study, it is shown that the surface protein SasG, identified previously by analysis of genome sequences, and two homologous proteins, Pls of and AAP of , also promote bacterial adherence to nasal epithelial cells. Conditions for expression of SasG by were not found. Adherence assays were therefore performed with and expressing SasG from an expression plasmid. These studies showed that SasG did not bind several ligands typically bound by . Significantly, SasG and Pls did promote bacterial adherence to nasal epithelial cells. Furthermore, pre-incubation of epithelial cells with purified recombinant proteins revealed that the N-terminal A regions of SasG, Pls and AAP, but not the B repeats of SasG, inhibited adherence of expressing SasG in a dose-dependent fashion. These results suggest that SasG, Pls and AAP bind to the same as-yet-unidentified receptor on the surface of nasal epithelial cells. In addition, expression of SasG, like Pls, reduced adherence of to fibronectin and fibrinogen.

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2003-10-01
2019-11-22
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References

  1. Aly, R., Shinefield, H. I., Strauss, W. G. & Maibach, H. I. ( 1977; ). Bacterial adherence to nasal mucosal cells. Infect Immun 17, 546–549.
    [Google Scholar]
  2. Bibel, D. J., Aly, R., Shinefield, H. R., Maibach, H. I. & Strauss, W. G. ( 1982; ). Importance of the keratinized epithelial cell in bacterial adherence. J Invest Dermatol 79, 250–253.[CrossRef]
    [Google Scholar]
  3. Cole, A. M., Tahk, S., Oren, A., Yoshioka, D., Kim, Y.-H., Park, A. & Ganz, T. ( 2001; ). Determinants of Staphylococcus aureus nasal carriage. Clin Diagn Lab Immunol 8, 1064–1069.
    [Google Scholar]
  4. Enright, M. C., Day, N. P. J., Davies, C. E., Peacock, S. J. & Spratt, B. G. ( 2000; ). Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol 38, 1008–1015.
    [Google Scholar]
  5. Foster, T. J. ( 1998; ). Molecular genetic analysis of staphylococcal virulence. Methods Microbiol 27, 433–454.
    [Google Scholar]
  6. Foster, T. J. & Höök, M. ( 1998; ). Surface protein adhesins of Staphylococcus aureus. Trends Microbiol 6, 484–488.[CrossRef]
    [Google Scholar]
  7. Gasson, M. J. ( 1983; ). Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing. J Bacteriol 154, 1–9.
    [Google Scholar]
  8. Hartford, O., Francois, P., Vaudaux, P. & Foster, T. J. ( 1997; ). The dipeptide repeat of the fibrinogen-binding protein (clumping factor) is required for functional expression of the fibrinogen-binding domain on the Staphylococcus aureus cell surface. Mol Microbiol 25, 1065–1076.[CrossRef]
    [Google Scholar]
  9. Hartford, O., O'Brien, L., Schofield, K., Wells, J. & Foster, T. J. ( 2001; ). The Fbe (SdrG) protein of Staphylococcus epidermidis HB promotes adherence to fibrinogen. Microbiology 147, 2545–2552.
    [Google Scholar]
  10. Hildén, P., Savolainen, K., Tyynelä, J., Vuento, M. & Kuusela, P. ( 1996; ). Purification and characterisation of a plasmin-sensitive surface protein of Staphylococcus aureus. Eur J Biochem 236, 904–910.[CrossRef]
    [Google Scholar]
  11. Huesca, M., Peralta, R., Sauder, D. N., Simor, A. E. & McGavin, M. J. ( 2002; ). Adhesion and virulence properties of epidemic Canadian methicillin-resistant Staphylococcus aureus strain 1: identification of novel adhesion functions associated with plasmin-sensitive surface protein. J Infect Dis 185, 1285–1296.[CrossRef]
    [Google Scholar]
  12. Hussain, M., Herrmann, M., von Eiff, C., Perdreau-Remington, F. & Peters, G. ( 1997; ). A 140-kilodalton extracellular protein is essential for the accumulation of Staphylococcus epidermidis strains on surfaces. Infect Immun 65, 519–524.
    [Google Scholar]
  13. Josefsson, E., O'Connell, D., Foster, T. J., Durussel, I. & Cox, J. A. ( 1998; ). The binding of calcium to the B-repeat segment of SdrD, a cell surface protein of Staphylococcus aureus. J Biol Chem 273, 31145–31152.[CrossRef]
    [Google Scholar]
  14. Kalinin, A., Marekov, L. N. & Steinert, P. M. ( 2001; ). Assembly of the epidermal cornified cell envelope. J Cell Sci 114, 3069–3070.
    [Google Scholar]
  15. Kluytmans, J., van Belkum, A. & Verbrugh, H. ( 1997; ). Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks. Clin Microbiol Rev 10, 505–520.
    [Google Scholar]
  16. Laemmli, U. K. ( 1970; ). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.[CrossRef]
    [Google Scholar]
  17. Lina, G., Boutite, F., Tristan, A., Bes, M., Etienne, J. & Vandenesch, F. ( 2003; ). Bacterial competition for human nasal cavity colonization: role of staphylococcal agr alleles. Appl Environ Microbiol 69, 18–23.[CrossRef]
    [Google Scholar]
  18. Luzar, M. A., Coles, G. A., Faller, B. & 7 other authors ( 1990; ). Staphylococcus aureus nasal carriage and infection in patients on continuous ambulatory peritoneal dialysis. N Engl J Med 322, 505–509.[CrossRef]
    [Google Scholar]
  19. Mazmanian, S. K., Ton-That, H. & Schneewind, O. ( 2001; ). Sortase-catalysed anchoring of surface proteins to the cell wall of Staphylococcus aureus. Mol Microbiol 40, 1049–1057.[CrossRef]
    [Google Scholar]
  20. McAleese, F. M., Walsh, E. J., Sieprawska, M., Potempa, J. & Foster, T. J. ( 2001; ). Loss of clumping factor B fibrinogen binding activity by Staphylococcus aureus involves cessation of transcription, shedding and cleavage by metalloprotease. J Biol Chem 276, 29969–29978.[CrossRef]
    [Google Scholar]
  21. Navarre, W. W. & Schneewind, O. ( 1999; ). Surface proteins of Gram-positive bacteria and mechanisms of their targeting to the cell wall envelope. Microbiol Mol Biol Rev 63, 174–229.
    [Google Scholar]
  22. Ní Eidhin, D., Perkins, S., Francois, P., Vaudaux, P., Höök, M. & Foster, T. J. ( 1998; ). Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin of Staphylococcus aureus. Mol Microbiol 30, 245–257.[CrossRef]
    [Google Scholar]
  23. Novick, R. P. ( 1967; ). Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology 33, 155–166.[CrossRef]
    [Google Scholar]
  24. O'Brien, L. M., Walsh, E. J., Massey, R. C., Peacock, S. J. & Foster, T. J. ( 2002; ). Staphylococcus aureus clumping factor B (ClfB) promotes adherence to human type I cytokeratin 10: implications for nasal colonization. Cell Microbiol 4, 759–770.[CrossRef]
    [Google Scholar]
  25. Owen, P. ( 1985; ). Crossed immunoelectrophoresis in the study of outer membrane antigens. In Enterobacterial Surface Antigens: Methods for Molecular Characterization, pp. 207–242. Edited by T. K. K. Korhonen, E. A. Dawes & P. H. Makela. Amsterdam: Elsevier.
  26. Peacock, S. J., de Silva, I. & Lowy, F. D. ( 2001; ). What determines nasal carriage of Staphylococcus aureus? Trends Microbiol 9, 605–610.[CrossRef]
    [Google Scholar]
  27. Perkins, S., Walsh, E. J., Deivanayagam, C. C. S., Narayana, S. V. L., Foster, T. J. & Höök, M. ( 2001; ). Structural organization of the fibrinogen-binding region of the clumping factor B MSCRAMM of Staphylococcus aureus. J Biol Chem 276, 44721–44728.[CrossRef]
    [Google Scholar]
  28. Pirisi, L., Creek, K. E., Doniger, J. & DiPaola, J. A. ( 1988; ). Continuous cell lines with altered growth and differentiation properties originate after transfection of human keratinocytes with human papillomavirus type 16 DNA. Carcinogenesis 9, 1573–1579.[CrossRef]
    [Google Scholar]
  29. Roche, F. M., Massey, R., Peacock, S. J., Day, N. P. J., Visai, L., Speziale, P., Lam, A., Pallen, M. & Foster, T. J. ( 2003; ). Characterization of novel LPXTG-containing proteins of Staphylococcus aureus identified from genome sequences. Microbiology 149, 643–654.[CrossRef]
    [Google Scholar]
  30. Saravia-Otten, P., Müller, H.-P. & Arvidson, S. ( 1997; ). Transcription of Staphylococcus aureus fibronectin binding protein genes is negatively regulated by agr and an agr-independent mechanism. J Bacteriol 179, 5259–5263.
    [Google Scholar]
  31. Savolainen, K., Paulin, L., Westerlund-Wikström, B., Foster, T. J., Korhonen, T. K. & Kuusela, P. ( 2001; ). Expression of pls, a gene closely associated with the mecA gene of methicillin-resistant Staphylococcus aureus, prevents bacterial adhesion in vitro. Infect Immun 69, 3013–3020.[CrossRef]
    [Google Scholar]
  32. Shafer, W. M. & Iandolo, J. J. ( 1979; ). Genetics of staphylococcal enterotoxin B in methicillin-resistant isolates of Staphylococcus aureus. Infect Immun 25, 902–911.
    [Google Scholar]
  33. Wann, E. R., Gurusiddappa, S. & Höök, M. ( 2000; ). The fibronectin-binding MSCRAMM FnbpA of Staphylococcus aureus is a bifunctional protein that also binds to fibrinogen. J Biol Chem 275, 13863–13871.[CrossRef]
    [Google Scholar]
  34. Wells, J. M. & Schofield, K. M. ( 1996; ). Cloning and expression vectors for lactococci. In Lactic Acid Bacteria: Current Advances in Metabolism, Genetics and Application, Nato ASI series, vol. H98, pp. 37–62. Edited by T. F. Bozoglu & B. Ray. Berlin: Springer.
  35. Wells, J. M., Wilson, P. W. & Le Page, R. W. F. ( 1993; ). Improved cloning vectors and transformation procedure for Lactococcus lactis. J Appl Bacteriol 74, 629–636.[CrossRef]
    [Google Scholar]
  36. Yu, V. L., Goetz, A., Wagener, M., Smith, P. B., Rihs, J. D., Hanchett, J. & Zuravleff, J. J. ( 1986; ). Staphylococcus aureus nasal carriage and infection in patients on hemodialysis. Efficacy of antibiotic prophylaxis. N Engl J Med 315, 91–96.[CrossRef]
    [Google Scholar]
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