1887

Abstract

To study the periplasmic branch of iron (ferric ion) uptake systems in Gram-negative bacteria, genetic reconstitution experiments were initiated in involving exchange of the periplasmic iron-binding protein. The expression of many of the heterologous periplasmic ferric-binding proteins (FbpAs) was quite limited. Transformation experiments with the gene from yielded two colony sizes with different phenotypic characteristics. The small colonies contained the intact gene and were deficient in utilization of transferrin iron. The large colonies contained hybrid / genes, were proficient in transferrin iron utilization and had enhanced levels of expression of FbpA. These hybrid genes included several that encoded the mature FbpA with the signal peptide. To more fully evaluate the effect of foreign signal peptides, a series of hybrid genes were prepared that exchanged the signal peptides from FbpA, FbpA and the TEM-1 -lactamase. The presence of the leader was required for functional expression of FbpAs and was shown to dramatically increase the level of -lactamase activity.

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2003-11-01
2019-10-22
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