1887

Abstract

The phosphoenolpyruvate : sugar phosphotransferase system (PTS) catalyses translocation with concomitant phosphorylation of sugars and hexitols and it regulates metabolism in response to the availability of carbohydrates. The PTS forms an interface between energy and signal transduction and its inhibition is likely to have pleiotropic effects. It is present in about one-third of bacteria with fully sequenced genomes, including many common pathogens, but does not occur in eukaryotes. Enzyme I () is the first component of the divergent protein phosphorylation cascade. deletions were constructed in , and and virulence of the mutants was characterized in an intraperitoneal mouse model. The log(attenuation) values were 2·3, 1·4 and 0·9 for the , and mutants, respectively. The degree of attenuation is correlated with the complexity of the respective PTS, which comprises approximately 40 components in , but only 5 in .

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2003-09-01
2020-08-08
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References

  1. Barcak G. J., Chandler M. S., Redfield R. J., Tomb J. F.. 1991; Genetic systems in Haemophilus influenzae . Methods Enzymol204:321–342
    [Google Scholar]
  2. Bishai W. R., Howard N. S., Winkelstein J. A., Smith H. O.. 1994; Characterization and virulence analysis of catalase mutants of Haemophilus influenzae . Infect Immun62:4855–4860
    [Google Scholar]
  3. Brodeur B. R., Tsang P. S., Hamel J., Larose Y., Montplaisir S.. 1986; Mouse models of infection for Neisseria meningitidis B, 2b and Haemophilus influenzae type b diseases. Can J Microbiol32:33–37
    [Google Scholar]
  4. Bruckner R.. 1997; Gene replacement in Staphylococcus carnosus and Staphylococcus xylosus . FEMS Microbiol Lett151:1–8
    [Google Scholar]
  5. Buchmeier N. A., Lipps C. J., So M. Y., Heffron F.. 1993; Recombination-deficient mutants of Salmonella typhimurium are avirulent and sensitive to the oxidative burst of macrophages. Mol Microbiol7:933–936
    [Google Scholar]
  6. Chang A. C., Cohen S. N.. 1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol134:1141–1156
    [Google Scholar]
  7. Chatfield S. N., Dorman C. J., Hayward C., Dougan G.. 1991; Role of ompR -dependent genes in Salmonella typhimurium virulence: mutants deficient in both ompC and ompF are attenuated in vivo . Infect Immun59:449–452
    [Google Scholar]
  8. Coulter S. N., Schwan W. R., Ng E. Y. W.. 7 other authors 1998; Staphylococcus aureus genetic loci impacting growth and survival in multiple infection environments. Mol Microbiol30:393–404
    [Google Scholar]
  9. Curtiss R. III, Kelly S. M.. 1987; Salmonella typhimurium deletion mutants lacking adenylate cyclase and cyclic AMP receptor protein are avirulent and immunogenic. Infect Immun55:3035–3043
    [Google Scholar]
  10. De Souza-Hart J. A., Blackstock W., Di Modugno V., Holland I. B., Kok M.. 2003; Two-component systems in Haemophilus influenzae : a regulatory role for ArcA in serum resistance. Infect Immun71:163–172
    [Google Scholar]
  11. Edelstein P. H., Edelstein M. A., Higa F., Falkow S.. 1999; Discovery of virulence genes of Legionella pneumophila by using signature tagged mutagenesis in a guinea pig pneumonia model. Proc Natl Acad Sci U S A96:8190–8195
    [Google Scholar]
  12. Erni B., Zanolari B., Kocher H. P.. 1987; The mannose permease of Escherichia coli consists of three different proteins: amino-acid sequence and function in sugar transport, sugar phosphorylation, and penetration of phage lambda DNA. J Biol Chem262:5238–5247
    [Google Scholar]
  13. Fleischmann R. D., Adams M. D., White O.. 37 other authors 1995; Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science269:496–512
    [Google Scholar]
  14. Fomenkov A., Valiakhmetov A., Brand L., Roseman S.. 1998; In vivo and in vitro complementation of the N-terminal domain of enzyme I of the Escherichia coli phosphotransferase system by the cloned C-terminal domain. Proc Natl Acad Sci U S A95:8491–8495
    [Google Scholar]
  15. Furste J. P., Pansegrau W., Frank R., Blocker H., Scholz P., Bagdasarian M., Lanka E.. 1986; Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene48:119–131
    [Google Scholar]
  16. Hava D., Camilli A.. 2002; Large-scale identification of serotype 4 Streptococcus pneumoniae virulence factors. Mol Microbiol45:1389–1406
    [Google Scholar]
  17. Jarosik G. P., Sanders J. D., Cope L. D., Muller-Eberhard U., Hansen E. J.. 1994; A functional tonB gene is required for both utilization of heme and virulence expression by Haemophilus influenzae type b. Infect Immun62:2470–2477
    [Google Scholar]
  18. Jones A. L., Knoll K. M., Rubens C. E.. 2000; Identification of Streptococcus agalactiae virulence genes in the neonatal rat sepsis model using signature-tagged mutagenesis. Mol Microbiol37:1444–1455
    [Google Scholar]
  19. Khan M. A., Isaacson R. E.. 1998; In vivo expression of the β -glucoside (bgl) operon of Escherichia coli occurs in mouse liver. J Bacteriol180:4746–4749
    [Google Scholar]
  20. Lau G. W., Haataja S., Lonetto M., Kensit S. E., Marra A., Bryant A. P., McDevitt D., Morrison D. A., Holden D. W.. 2001; A functional genomic analysis of type 3 Streptococcus pneumoniae virulence. Mol Microbiol40:555–571
    [Google Scholar]
  21. LiCalsi C., Crocenzi T. S., Freire E., Roseman S.. 1991; Sugar transport by the bacterial phosphotransferase system. Structural and thermodynamic domains of Enzyme I of Salmonella typhimurium . J Biol Chem266:19519–19527
    [Google Scholar]
  22. Lowe A. M., Beattie D. T., Deresiewicz R. L.. 1998; Identification of novel staphylococcal virulence genes by in vivo expression technology. Mol Microbiol27:967–976
    [Google Scholar]
  23. Lowy F. D.. 1998; Staphylococcus aureus infections. N Engl J Med339:520–532
    [Google Scholar]
  24. Lycheva T. A., Bondarenko V. M., Burd G. I., Umiarov A. M., Bol'shakova T. N.. 1980; Characteristics of attenuated immunogenic mutants of Shigella flexneri selected from phosphomycin-resistant clones. Zh Mikrobiol Epidemiol Immunobiol June58–63 in Russian
    [Google Scholar]
  25. Mahan M. J., Tobias J. W., Slauch J. M., Hanna P. C., Collier R. J., Mekalanos J. J.. 1995; Antibiotic-based selection for bacterial genes that are specifically induced during infection of a host. Proc Natl Acad Sci U S A92:669–673
    [Google Scholar]
  26. Miller S. I., Mekalanos J. J.. 1990; Constitutive expression of the phoP regulon attenuates Salmonella virulence and survival within macrophages. J Bacteriol172:2485–2490
    [Google Scholar]
  27. Morse M. L., Alire M. L.. 1958; An agar medium indicating acid production. J Bacteriol177:6144–6152
    [Google Scholar]
  28. Moxon E. R., Kroll J. S.. 1988; Type b capsular polysaccharide as a virulence factor of Haemophilus influenzae . Vaccine6:113–115
    [Google Scholar]
  29. Paulsen I. T., Sliwinski M. K., Saier M. H.. 1998; Microbial genome analyses: global comparisons of transport capabilities based on phylogenies, bioenergetics and substrate specificities. J Mol Biol277:573–592
    [Google Scholar]
  30. Postma P. W., Lengeler J. W., Jacobson G. R.. others 1996; Phosphoenolpyruvate : carbohydrate phosphotransferase systems. In Escherichia coli and Salmonella: Cellular and Molecular Biology pp1149–1174 Edited by Neidhardt F. C.. Washington, DC: American Society for Microbiology;
  31. Powell B. S., Court D. L., Inada T., Nakamura Y., Michotey V., Cui X., Reizer A., Saier M. H., Reizer J.. 1995; Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli . Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an era ts mutant. J Biol Chem270:4822–4839
    [Google Scholar]
  32. Priefer U. B., Simon R., Puhler A.. 1985; Extension of the host range of Escherichia coli vectors by incorporation of RSF1010 replication and mobilization functions. J Bacteriol163:324–330
    [Google Scholar]
  33. Reed L. J., Muench H.. 1938; A simple method of estimating fifty percent endpoints. Am J Hyg27:493–497
    [Google Scholar]
  34. Saier M. H. Jr, Chin A. M.. 1990; Energetics of the bacterial phosphotransferase system in sugar transport and regulation of carbon metabolism. In Bacterial Energetics pp273–299 Edited by Krulwich T. A. San Diego, CA: Academic Press;
  35. Sambrook J., Fritsch E. F., Maniatis T.. 1989; Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  36. Sharples G. J.. 1996; Haemophilus virulence. Microbiology142:717
    [Google Scholar]
  37. Stuy J. H., Walter R. B.. 1986; Homology-facilitated plasmid transfer in Haemophilus influenzae . Mol Gen Genet203:288–295
    [Google Scholar]
  38. Turner A. K., Lovell M. A., Hulme S. D., Zhang-Barber L., Barrow P. A.. 1998; Identification of Salmonella typhimurium genes required for colonization of the chicken alimentary tract and for virulence in newly hatched chicks. Infect Immun66:2099–2106
    [Google Scholar]
  39. Van der Vlag J., Van't Hof R., Van Dam K., Postma P. W.. 1995; Control of glucose metabolism by the enzymes of the glucose phosphotransferase system in Salmonella typhimurium . Eur J Biochem230:170–182
    [Google Scholar]
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