1887

Abstract

Cholic acid (CA) transport was investigated in nine intestinal strains. Upon energization with glucose, all of the bifidobacteria accumulated CA. The driving force behind CA accumulation was found to be the transmembrane proton gradient (ΔpH, alkaline interior). The levels of accumulated CA generally coincided with the theoretical values, which were calculated by the Henderson–Hasselbalch equation using the measured internal pH values of the bifidobacteria, and a p value of 6·4 for CA. These results suggest that the mechanism of CA accumulation is based on the diffusion of a hydrophobic weak acid across the bacterial cell membrane, and its dissociation according to the ΔpH value. A mixture of short-chain fatty acids (acetate, propionate and butyrate) at the appropriate colonic concentration (117 mM in total) reduced CA accumulation in JCM 1192. These short-chain fatty acids, which are weak acids, reduced the ΔpH, thereby decreasing CA accumulation in a dose-dependent manner. The bifidobacteria did not alter or modify the CA molecule. The probiotic potential of CA accumulation is discussed in relation to human bile acid metabolism.

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2003-08-01
2020-05-27
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References

  1. Baron S. F., Hylemon P. B.. 1997; Biotransformation of bile acids, cholesterol, and steroid hormones. In Gastrointestinal Microbiology, vol. 1, Gastrointestinal Ecosystems and Fermentations pp470–510 Edited by Mackie R. I., White B. A.. New York: International Thomson Publishing;
    [Google Scholar]
  2. Binder H. J., Filburn B., Floch M.. 1975; Bile acid inhibition of intestinal anaerobic organisms. Am J Clin Nutr28:119–125
    [Google Scholar]
  3. Breeuwer P., Drocourt J., Rombouts F. M., Abee T.. 1996; A novel method for continuous determination of the intracellular pH in bacteria with the internally conjugated fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester. Appl Environ Microbiol62:178–183
    [Google Scholar]
  4. Cummings J. H.. 1997; Short-chain fatty acids. In The Large Intestine in Nutrition and Disease pp43–65 Bruxelles: Institut Danone;
    [Google Scholar]
  5. Cummings J. H., Macfarlane G. T.. 1997; Role of intestinal bacteria in nutrient metabolism. Clin Nutr16:3–11
    [Google Scholar]
  6. Cummings J. H., Macfarlane G. T., Englyst H. N.. 2001; Prebiotic digestion and fermentation. Am J Clin Nutr73:415S–420S
    [Google Scholar]
  7. Diez-Gonzalez F., Russell J. B.. 1997; Effects of carbonylcyanide- m -chlorophenylhydrazone (CCCP) and acetate on Escherichia coli O157 : H7 and K-12: uncoupling versus anion accumulation. FEMS Microbiol Lett151:71–76
    [Google Scholar]
  8. Elkins C. A., Savage D. C.. 1998; Identification of genes encoding conjugated bile salt hydrolase and transport in Lactobacillus johnsonii 100-100. J Bacteriol180:4344–4349
    [Google Scholar]
  9. Elkins C. A., Moser S. A., Savage D. C.. 2001; Genes encoding bile salt hydrolases and conjugated bile salt transporters in Lactobacillus johnsonii 100-100 and other Lactobacillus species. Microbiology147:3403–3412
    [Google Scholar]
  10. Eneroth P.. 1963; Thin-layer chromatography of bile acids. J Lipid Res4:11–16
    [Google Scholar]
  11. Ewe K., Karbach U.. 1989; Functions of the alimentary canal. In Human Physiology pp693–734 Edited by Schmidt R. F., Thews G. Berlin: Springer-Verlag;
    [Google Scholar]
  12. Kamp F., Hamilton J. A.. 1993; Movement of fatty acids, fatty acid analogues, and bile acids across phospholipid bilayers. Biochemistry32:11074–11086
    [Google Scholar]
  13. Kitahara M., Takamine F., Imamura T., Benno Y.. 2000; Assignment of Eubacterium sp. VPI 12708 and related strains with high bile acid 7 α -dehydroxylating activity to Clostridium scindens and proposal of Clostridium hylemonae sp. nov., isolated from human faeces. Int J Syst Evol Microbiol50:971–978
    [Google Scholar]
  14. Kurdi P., van Veen H. W., Tanaka H., Mierau I., Konings W. N., Tannock G. W., Tomita F., Yokota A.. 2000; Cholic acid is accumulated spontaneously, driven by membrane ΔpH, in many lactobacilli. J Bacteriol182:6525–6528
    [Google Scholar]
  15. Mallonee D. H., Hylemon P. B.. 1996; Sequencing and expression of a gene encoding a bile acid transporter from Eubacterium sp. strain VPI 12708. J Bacteriol178:7053–7058
    [Google Scholar]
  16. Masuda N.. 1981; Deconjugation of bile salts by Bacteroides and Clostridium . Microbiol Immunol25:1–11
    [Google Scholar]
  17. Mitsuoka T.. 2002; Prebiotics and intestinal flora. Biosci Microflora21:3–12
    [Google Scholar]
  18. Ouwehand A. C., Salminen S., Isolauri E.. 2002; Probiotics: an overview of beneficial effects. Antonie van Leeuwenhoek82:279–289
    [Google Scholar]
  19. Poolman B., Hellingwerf K. J., Konings W. N.. 1987; Regulation of the glutamate–glutamine transport system by intracellular pH in Streptococcus lactis . J Bacteriol169:2272–2276
    [Google Scholar]
  20. Reddy B. S., Watanabe K.. 1979; Effect of cholesterol metabolites and promoting effect of lithocholic acid in colon carcinogenesis in germ-free and conventional F344 rats. Cancer Res39:1521–1524
    [Google Scholar]
  21. Reddy B. S., Narasawa T., Weisburger J. H., Wynder E. L.. 1976; Promoting effect of sodium deoxycholate on colon adenocarcinomas in germfree rats. J Natl Cancer Inst56:441–442
    [Google Scholar]
  22. Russell J. B.. 1991; Resistance of Streptococcus bovis to acetic acid at low pH: relationship between intracellular pH and anion accumulation. Appl Environ Microbiol57:255–259
    [Google Scholar]
  23. Takahashi T., Morotomi M.. 1994; Absence of cholic acid 7 α -dehydroxylase activity in the strains of Lactobacillus and Bifidobacterium . J Dairy Sci77:3275–3286
    [Google Scholar]
  24. Tanaka H., Doesburg k., Iwasaki T., Mierau I.. 1999; Screening of lactic acid bacteria for bile salt hydrolase activity. J Dairy Sci82:2530–2535
    [Google Scholar]
  25. Tanaka H., Hashiba H., Kok J., Mierau I.. 2000; Bile salt hydrolase of Bifidobacterium longum – biochemical and genetic characterization. Appl Environ Microbiol66:2502–2512
    [Google Scholar]
  26. Thanassi D. G., Cheng L. W., Nikaido H.. 1997; Active efflux of bile salts by Escherichia coli . J Bacteriol179:2512–2518
    [Google Scholar]
  27. White B. A., Lipsky R. L., Fricke R. J., Hylemon P. B.. 1980; Bile acid induction specificity of 7 α -dehydroxylase activity in an intestinal Eubacterium species. Steroids35:103–109
    [Google Scholar]
  28. Yokota A., Veenstra M., Kurdi P., van Veen H. W., Konings W. N.. 2000; Cholate resistance in Lactococcus lactis is mediated by an ATP-dependent multispecific organic anion transporter. J Bacteriol182:5196–5201
    [Google Scholar]
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