1887

Abstract

, the causative agent of chestnut blight, has proven to be a tractable experimental system for studying fungal pathogenesis. Moreover, the development of infectious cDNA clones of hypoviruses, capable of attenuating fungal virulence, has provided the opportunity to examine molecular aspects of fungal plant pathogenesis in the context of biological control. In order to establish a genomic base for future studies of , the authors have analysed a collection of expressed sequences. A mixed cDNA library was prepared from RNA isolated from wild-type (virus-free) and hypovirus-infected strains. Plasmid DNA was recovered from individual transformants and sequenced from the 5′ end of the insert. Contig analysis of the collected sequences revealed that they represented approximately 2200 individual ORFs. An assessment of functional diversity present in this collection was achieved by using the software utilities and the NCBI protein database. Candidate genes were identified with significant potential relevance to growth, development, pathogenesis and vegetative incompatibility. Additional investigations of a 12·9 kbp genomic region revealed microsynteny between and both sa and , two closely related fungi. These data represent the largest collection of sequence information currently available for and are now forming the basis of further studies using microarray analyses to determine global changes in transcription that occur in response to hypovirus infection.

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2003-09-01
2019-11-14
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Supplementary Table A. A redundant list in order of clone identification number of the BLAST results for clones that gave hits of 1x10 or smaller and classified according to Molecular Function.

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Supplementary Table B. A redundant list in order of clone identification number of the BLAST results for clones that gave hits of 1x10 or smaller and classified according to Biological Process.

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