1887

Abstract

Treatment of bacterial cultures with chelating agents such as 2,2′-dipyridyl (DPD) induces expression of iron-regulated genes. It is known that in the -, the Fur protein is the major regulator of genes encoding haem- or haemoglobin-binding proteins. Electrophoretic analysis of outer-membrane proteins of the -proteobacterium has revealed the induction of two proteins of 60 and 40 kDa in DPD-treated cultures in both wild-type and -defective strains. These two proteins have the same N-terminal amino acid sequence, which identifies this protein as the product of the PM0592 ORF. Analysis of the sequence of this ORF, which encodes a protein of 60 kDa, revealed the presence of a hexanucleotide (AAAAAA) at which a programmed translational frameshift can occur giving rise to a 40 kDa protein. Analyses conducted in , using the complete PM0592 ORF and a derivative truncated at the hexanucleotide position, have shown that both polypeptides bind haemin. For this reason, the PM0592 ORF product has been designated HbpA (for aemin-inding rotein). Expression studies using both RT-PCR and fusions, as well as electrophoretic profiles of outer-membrane protein composition, have demonstrated that the gene is negatively regulated by iron, manganese and haemin through a -independent pathway. Despite the fact that serum of mice infected with contained antibodies that reacted with both the 60 and 40 kDa products of the gene, these proteins did not offer protection when used in immunization assays against this micro-organism.

Keyword(s): DPD, 2,2′-dipyridyl
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2003-08-01
2020-10-20
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