1887

Abstract

The authors have previously shown that overexpression of the K-12 , and genes protects the chromosome from decondensation by camphor. In this study they examine the phenotypic consequences of deleting or overexpressing , and . Overexpressing , and increases supercoiling levels of plasmids in wild-type cells and in temperature-sensitive (Ts) gyrase mutants, suppresses the sensitivity of gyrase and topoisomerase IV (topo IV) Ts mutants to nalidixic acid, makes gyrase and topo IV Ts mutants more resistant to camphor and corrects the nucleoid morphology defects in topo IV Ts mutants. Overexpression of , and results in a slight (2·2-fold) activation of the gene. Deleting , and is not lethal to cells but results in an increase in sensitivity to camphor. Deletion of , and exacerbates the nucleoid morphology defects of the topo IV Ts mutants. When the individual , or genes were tested for their effects on camphor resistance and regulation of , alone conferred 10-fold camphor resistance and 1·7-fold activation of . These activities were augmented when was overexpressed with (100-fold camphor resistance and 2·1-fold induction of ).

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2003-08-01
2019-10-20
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