1887

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Volume 149, Issue 9

Glucose and trehalose PTS permeases of probably share a single IIA domain, enabling the spiroplasma to adapt quickly to carbohydrate changes in its environment Free

Abstract

is a plant-pathogenic mollicute phylogenetically related to Gram-positive bacteria. Spiroplasma cells are restricted to the phloem sieve tubes and are transmitted from plant to plant by the leafhopper vector . In the plant sieve tubes, grows on glucose and fructose, whereas in the leafhopper haemolymph the spiroplasma must grow on trehalose, the major sugar in insects. Previous studies in this laboratory have shown that fructose utilization was a key factor of spiroplasmal pathogenicity. To further study the implication of sugar metabolism in the interactions of with its plant host and its leafhopper vector, genes encoding permease enzymes II (EII and EII) of the phosphoenolpyruvate : glucose and phosphoenolpyruvate : trehalose phosphotransferase systems (PTS) were characterized. Mapping studies revealed that the EII complex was split into two distinct polypeptides, IIA and IICB, encoded by two separate genes, and , respectively. As expected, polypeptides IIA and IICB were more phylogenetically related to their counterparts from Gram-positive than to those from Gram-negative bacteria. The trehalose operon consisted of three genes , and , encoding a transcriptional regulator, the PTS permease (EII) and the amylase, respectively. However, in contrast to the fructose-PTS permease, which is encoded as a single polypeptide (IIABC) containing the three domains A, B and C, the trehalose-PTS permease (IIBC) lacks its own IIA domain. No trehalose-specific IIA could be identified in the spiroplasmal genome, suggesting that the IIBC permease probably functions with the IIA domain. In agreement with this statement, yeast two-hybrid system experiments revealed that the IIA domain interacted not only with IIB but also with the IIB domain. The results are discussed with respect to the ability of the spiroplasma to adapt from the phloem sap of the host plant to the haemolymph and salivary gland cells of the insect vector.

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Keyword(s): PTS, phosphoenolpyruvate : sugar phosphotransferase system
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2003-09-01
2024-04-20
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Glucose and trehalose PTS permeases of Spiroplasma citri probably share a single IIA domain, enabling the spiroplasma to adapt quickly to carbohydrate changes in its environment
Microbiology 149, 2687 (2003); https://doi.org/10.1099/mic.0.26336-0
/content/journal/micro/10.1099/mic.0.26336-0
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