1887

Abstract

The methylotrophic yeast exhibits -formylglutathione hydrolase activity (FGH, EC 3.1.2.12), which is involved in the glutathione-dependent formaldehyde oxidation pathway during growth on methanol as the sole carbon source. The structural gene, , was cloned from , and its predicted amino acid sequence showed more than 60 % similarity to those of FGHs from and , and human esterase D. FGH from contained a C-terminal tripeptide, SKL, which is a type I peroxisome-targeting signal, and a bimodal distribution of FGH between peroxisomes and the cytosol was demonstrated. The gene was disrupted in the genome by one-step gene disruption. The Δ strain was still able to grow on methanol as a carbon source under methanol-limited chemostat conditions with low dilution rates (<0·05 h), conditions under which a strain with disruption of the gene for formaldehyde dehydrogenase (another enzyme involved in the formaldehyde oxidation pathway) could not survive. These results suggested that FGH is not essential but necessary for optimal growth on methanol. This is believed to be the first report of detailed analyses of the gene in a methylotrophic yeast strain.

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2003-08-01
2019-10-18
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vol. , part 8, pp. 1971 - 1979

Fig. 2b is incorrect. The corrected figure is given below.

In the legend of Fig. 2, '(b) Genomic Southern analysis of I-digested total DNAs' should read '(b) Genomic Southern analysis of II-digested total DNAs'

In the last sentence of the Methods section (p. 1974, line 1, of the printed journal), 'using I-digested genomic DNA' should read 'using II-digested genomic DNA'



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