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Volume 149, Issue 11

Characterization of a soil-derived bacterial consortium degrading 4-chloroaniline Free

Abstract

A bacterial consortium comprising four different species was isolated from an Indonesian agricultural soil using a mixture of aniline and 4-chloroaniline (4CA) as principal carbon sources. The four species were identified as SB1, SB2, SB4 and SB5. Growth studies on aniline and 4CA as single and mixed substrates demonstrated that the bacteria preferred to grow on and utilize aniline rather than 4CA, although both compounds were eventually depleted from the culture supernatant. However, despite 100 % disappearance of the parent substrates, the degradation of 4CA was always characterized by incomplete dechlorination and 4-chlorocatechol accumulation. This result suggests that further degradation of 4-chlorocatechol may be the rate-limiting step in the metabolism of 4CA by the bacterial consortium. HPLC-UV analysis showed that 4-chlorocatechol was further degraded via an -cleavage pathway by the bacterial consortium. This hypothesis was supported by the results from enzyme assays of the crude cell extract of the consortium revealing catechol 1,2-dioxygenase activity which converted catechol and 4-chlorocatechol to ,-muconic acid and 3-chloro-,-muconic acid respectively. However, the enzyme had a much higher conversion rate for catechol [156 U (g protein)] than for 4-chlorocatechol [17·2 U (g protein)], indicating preference for non-chlorinated substrates. Members of the bacterial consortium were also characterized individually. All isolates were able to assimilate aniline. SB4 was able to grow on 4CA solely, while SB5 was able to grow on 4-chlorocatechol. These results suggest that the degradation of 4CA in the presence of aniline by the bacterial consortium was a result of interspecies interactions.

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Keyword(s): 4CA, 4-chloroaniline and FAME, fatty acid methyl ester
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2003-11-01
2024-04-25
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Characterization of a soil-derived bacterial consortium degrading 4-chloroaniline
Microbiology 149, 3279 (2003); https://doi.org/10.1099/mic.0.26303-0
/content/journal/micro/10.1099/mic.0.26303-0
/content/journal/micro/10.1099/mic.0.26303-0
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