1887

Abstract

The strain A1501 (formerly known as ) fixes nitrogen under microaerobic conditions in the free-living state and colonizes rice endophytically. The authors characterized a region in strain A1501, corresponding to most of the genes and the genes, involved in electron transport to nitrogenase in . The region contained three groups of genes arranged in the same order as in : (1) ORF3 ORF5 ORF6; (2) /; (3) ORF13 ORF12- ORF1 ORF2-. Unlike in , where these genes are not contiguous on the chromosome, but broken into two regions of the genome, the genes characterized here in are contiguous and present on a 30 kb region in the genome of this organism. Insertion mutagenesis confirmed that most of the and the genes in A1501 were essential for nitrogen fixation. Using fusions it was found that and gene expression was under the control of , and and that the gene products were involved in the regulation of the nitrogen fixation process.

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2003-08-01
2019-10-22
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