1887

Abstract

Haem is required for optimal growth of the bacterial anaerobe . Previous studies have shown that growth in the presence of haem is coincident with increased yields of ATP from glucose, expression of -type cytochromes and expression of fumarate reductase activity. This paper describes the identification of the genes that encode the cytochrome, iron–sulfur cluster protein and flavoprotein of the fumarate reductase. These genes, , and , respectively, are organized in an operon. Nonpolar, in-frame deletions of and were constructed in the chromosome. These mutant strains had no detectable fumarate reductase or succinate dehydrogenase activity. In addition, the mutant strains showed a threefold increase in generation time, relative to the wild-type strain. Growth of these mutant strains was fully restored to the wild-type rate by the introduction of a replicon containing the entire operon. Growth of the mutant strains was partially restored by supplementing the growth medium with succinate, indicating that the gene products function as a fumarate reductase. During growth on glucose, the mutant strains showed a threefold decrease in cell mass yield, relative to the wild-type strain. These data indicate that fumarate reductase is important for both energy metabolism and succinate biosynthesis in .

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2003-06-01
2020-11-28
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