1887

Abstract

A comparative study on phospholipids of , and was carried out using fast-atom bombardment (FAB) and electrospray ionization (ESI) mass spectrometry. Data obtained indicate the presence of acylphosphatidylglycerol (APG), diphosphatidylglycerol, phosphatidylglycerol (PG), phosphatidylinositol (PI) and triacylphosphatidylinositol dimannosides (AcPIM) in these bacteria. In general, octadecenoyl and hexadecanoyl fatty acyl moieties predominated in phospholipids of , whereas high levels of hexadecenoyl were found in and . Mass spectra from purified APG and PG indicated that the -1 position of the glycerol was occupied by octadecenoyl in the three species studied. Notably, several major molecular species of PI and AcPIM from contained significant amounts of a moiety identified as 10-methyleneoctadecanoyl, located at the -1 position of these molecules. On the other hand, multiantibiotic resistant and susceptible strains of differed in several minor phospholipid fatty acids of 19 carbon atoms, identified as 10-methyloctadecenoic, 10-methyloctadecanoic (tuberculostearic acid) and 10-methyleneoctadecanoic. The results demonstrate an overall similarity among the phospholipids of the different species studied but also significant differences related to the acyl chains of the glycerol moiety of these compounds, notably the high levels of an unusual fatty acyl moiety in inositol-containing phospholipids of .

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2003-07-01
2019-10-19
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