1887

Abstract

The 8399 clinical isolate secretes dihydroxybenzoic acid (DHBA) and a high-affinity catechol siderophore, which is different from other bacterial iron chelators already characterized. Complementation assays with enterobactin-deficient strains led to the isolation of a cosmid clone containing 8399 genes required for the biosynthesis and activation of DHBA. Accordingly, the cloned fragment harbours a polycistronic operon encoding predicted proteins highly similar to several bacterial proteins required for DHBA biosynthesis from chorismic acid. Genes encoding deduced proteins related to the Fes and the DhbF proteins, and a putative phosphopantetheinyl transferase, all of them involved in the assembly and utilization of catechol siderophores in other bacteria, were found next to the locus. This 8399 gene cluster also contained the , and predicted genes encoding proteins potentially involved in transport of ferric siderophore complexes. The deduced products of the and genes are putative membrane proteins that belong to the RND and MFS efflux pump proteins, respectively. Interestingly, P45 is highly related to the P43 (EntS) protein that participates in the secretion of enterobactin. Although P114 is similar to other bacterial efflux pump proteins involved in antibiotic resistance, its genetic arrangement within this 8399 locus is different from that described in other bacteria. The product of is a Fur- and iron-regulated surface-exposed outer-membrane protein. These characteristics together with the presence of a predicted TonB box and its high similarity to other siderophore receptors indicate that OM73 plays such a role in 8399. The 184 nt intergenic region contains promoter elements that could drive the expression of these divergently transcribed genes, all of which are in close proximity to almost perfect Fur boxes. This arrangement explains the iron- and Fur-regulated expression of , and provides strong evidence for a similar regulation for the expression of .

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2003-05-01
2019-11-19
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