1887

Abstract

A 68 kDa fibronectin-binding protein (Fbp68) from displaying significant homology to several established or putative Fbps from other bacteria was identified. The one-copy gene is highly conserved in isolates. Fbp68 was expressed in in fusion with glutathione -transferase; the fusion protein and the native Fbp68 were purified. Immunoblot analysis and cell fractionation experiments revealed that Fbp68 is present on the surface of the bacteria. Far-immuno dot-blotting demonstrated that Fbp68 was capable of fixing fibronectin. Indirect immunofluorescence and ELISA were employed to demonstrate that could bind both soluble and immobilized fibronectin. With competitive adherence inhibition assays it was shown that antibodies raised against Fbp68 partially inhibited attachment of to fibronectin and Vero cells. Furthermore, Vero cells could fix purified membrane-immobilized Fbp68. Thus Fbp68 appears to be one of the several adhesins identified to date in .

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2003-10-01
2019-11-18
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