1887

Abstract

Among the seven species characterized within the genus , three (, and ) have so far been isolated from human flora and during infectious processes. Sequencing and analysis of 16S rDNA () has been described as the best method for identification of strains at the species level since phenotypic characteristics are unable to differentiate between species. sequencing for the three species isolated from humans showed more than 98 % identity between them. Four copies were found in the reference strains and in all the clinical isolates studied. The sequences of each were determined for the clinical strain ADV 360.1, and they showed a relatively high level of heterogeneity (1·43 %). In the majority of cases, polymorphic positions corresponded to nucleotides allowing differentiation between the three species isolated from humans. Moreover, variability observed between copies was higher than that between 16S rDNA sequences of and . Phylogenetic analysis showed that polymorphism between copies affected the position of strain ADV 360.1 in the tree. Variable positions occurred in stems and loops belonging to variable and hypervariable regions of the 16S rRNA secondary structure but did not change the overall structure of the 16S rRNA. PCR-RFLP experiments performed on 27 clinical isolates of sp. suggested that inter- heterogeneity occurs widely among the members of the genus . These results, together with the lack of phenotypic criteria for species differentiation, give preliminary arguments for unification of and .

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2003-06-01
2019-11-12
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