1887

Abstract

mutants were unaffected in Fe-dependent regulation of several operons that specify different Fe uptake systems, yet cloned partially corrected an mutant and Fur protein bound to canonical boxes. The lack of a phenotype in mutants is not due to functional redundancy with Irr, another member of the Fur superfamily found in the rhizobia, since double mutants are also unaffected in Fe-responsive regulation of several operons involved in Fe uptake. Neither Irr nor Fur is needed for symbiotic N fixation on peas. As in , mutants accumulated protoporphyrin IX. is not regulated by Fur and its Irr protein lacks the motif needed for haem-dependent post-translational modification that occurs in Irr. The similarities and differences in the Fur superfamily in the rhizobia and other Gram-negative bacteria are discussed.

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2003-05-01
2019-11-14
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