1887

Abstract

have been implicated recently in the pathogenesis of digital dermatitis (DD) and contagious ovine digital dermatitis (CODD) that are infectious diseases of bovine and ovine foot tissues, respectively. Previous analyses of treponemal 16S rDNA sequences, PCR-amplified directly from DD or CODD lesions, have suggested relatedness of animal to some human oral species isolated from periodontal tissues. In this study a range of adhesion and virulence-related properties of three animal isolates have been compared with representative human oral strains of and . In adhesion assays using biotinylated treponemal cells, cells bound in consistently higher numbers to fibronectin, laminin, collagen type I, gelatin, keratin and lactoferrin than did or animal isolates. However, animal DD strains adhered to fibrinogen at equivalent or greater levels than . All strains bound to the amino-terminal heparin I/fibrin I domain of fibronectin. 16S rDNA sequence analyses placed ovine strain UB1090 and bovine strain UB1467 within a cluster that was phylogenetically related to , while ovine strain UB1466 appeared more closely related to . These observations correlated with phenotypic properties. Thus, ATCC 35405, GM-1, and UB1466 had similar outer-membrane protein profiles, produced chymotrypsin-like protease (CTLP), trypsin-like protease and high levels of proline iminopeptidase, and co-aggregated with human oral bacteria and . Conversely, ATCC 35580, D2A-2, and animal strains UB1090 and UB1467 did not express CTLP or trypsin-like protease and did not co-aggregate with or . Taken collectively, these results suggest that human oral-related have broad host specificity and that similar control or preventive strategies might be developed for human and animal -associated infections.

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2003-05-01
2019-11-16
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