1887

Abstract

pSV1 is a plasmid in SANK95570 that carries the methylenomycin biosynthetic () gene cluster. An ordered cosmid map and an RI map have been constructed for pSV1, confirming that pSV1 is a 163 kb circular plasmid. The gene cluster has been found on three different replicon structures; the circular plasmid pSV1, the 356 kb linear plasmid SCP1 and, via SCP1 integration, the linear chromosome of A3(2). Comparison of pSV1 and SCP1 sequences revealed that the two plasmids have homology to each other only around the and regions, eliminating models in which pSV1 was generated by circularization of SCP1 or vice versa. It is likely that the gene cluster was horizontally transferred as a set together with the region in the comparatively recent evolutionary past.

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2003-05-01
2020-04-03
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