1887

Abstract

pSV1 is a plasmid in SANK95570 that carries the methylenomycin biosynthetic () gene cluster. An ordered cosmid map and an RI map have been constructed for pSV1, confirming that pSV1 is a 163 kb circular plasmid. The gene cluster has been found on three different replicon structures; the circular plasmid pSV1, the 356 kb linear plasmid SCP1 and, via SCP1 integration, the linear chromosome of A3(2). Comparison of pSV1 and SCP1 sequences revealed that the two plasmids have homology to each other only around the and regions, eliminating models in which pSV1 was generated by circularization of SCP1 or vice versa. It is likely that the gene cluster was horizontally transferred as a set together with the region in the comparatively recent evolutionary past.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.26102-0
2003-05-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/micro/149/5/mic1491351.html?itemId=/content/journal/micro/10.1099/mic.0.26102-0&mimeType=html&fmt=ahah

References

  1. Aguilar, A. & Hopwood, D. A. ( 1982; ). Determination of methylenomycin A synthesis by the pSV1 plasmid from Streptomyces violaceus-ruber SANK95570. J Gen Microbiol 128, 1893–1901.
    [Google Scholar]
  2. Bentley, S. D., Chater, K. F., Cerdeno-Tarraga, A. M. & 40 other authors ( 2002; ). Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature 417, 141–147.[CrossRef]
    [Google Scholar]
  3. Chater, K. F. & Bruton, C. J. ( 1985; ). Resistance, regulatory and production genes for the antibiotic methylenomycin are clustered. EMBO J 4, 1893–1897.
    [Google Scholar]
  4. Chu, G., Vollrath, D. & Davis, R. W. ( 1986; ). Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science 234, 1582–1585.[CrossRef]
    [Google Scholar]
  5. Evans, G. A., Lewis, K. & Rothenberg, B. E. ( 1989; ). High efficiency vectors for cosmid microcloning and genomic analysis. Gene 79, 9–20.[CrossRef]
    [Google Scholar]
  6. Hanafusa, T. & Kinashi, H. ( 1992; ). The structure of an integrated copy of the giant linear plasmid SCP1 in the chromosome of Streptomyces coelicolor 2612. Mol Gen Genet 231, 363–368.[CrossRef]
    [Google Scholar]
  7. Haneishi, T., Kitahara, N., Takiguchi, Y. & Arai, M. ( 1974; ). New antibiotics, methylenomycins A and B. I. Producing organism, fermentation and isolation, biological activities and physical and chemical properties. J Antibiot 27, 386–392.[CrossRef]
    [Google Scholar]
  8. Henderson, D. J., Lydiate, D. J. & Hopwood, D. A. ( 1989; ). Structural and functional analysis of the mini-circle, a transposable element of Streptomyces coelicolor A3(2). Mol Microbiol 3, 1307–1318.[CrossRef]
    [Google Scholar]
  9. Henderson, D. J., Brolle, D.-F., Kieser, T., Melton, R. E. & Hopwood, D. A. ( 1990; ). Transposition of IS117 (the Streptomyces coelicolor A3(2) mini-circle) to and from a cloned target site and into secondary chromosomal sites. Mol Gen Genet 224, 65–71.
    [Google Scholar]
  10. Hopwood, D. A. & Wright, H. M. ( 1976; ). Interaction of the plasmid SCP1 with the chromosome of Streptomyces coelicolor A3(2). In Second International Symposium on the Genetics of Industrial Microorganisms, pp. 607–619. Edited by K. D. MacDonald. London: Academic Press.
  11. Kinashi, H. ( 1994; ). Pulsed field gel electrophoresis: isolation and analysis of large linear plasmids. In Methods in Gene Technology, vol. 2, pp. 227–240. Edited by J. W. Dale & P. G. Sanders. London: JAI Press.
  12. Kinashi, H. & Shimaji-Murayama, M. ( 1991; ). Physical characterization of SCP1, a giant linear plasmid from Streptomyces coelicolor. J Bacteriol 173, 1523–1529.
    [Google Scholar]
  13. Kinashi, H., Shimaji, M. & Sakai, A. ( 1987; ). Giant linear plasmids in Streptomyces which code for antibiotic biosynthesis genes. Nature 328, 454–456.[CrossRef]
    [Google Scholar]
  14. Kinashi, H., Murayama, M., Matsushita, H. & Nimi, O. ( 1993; ). Structural analysis of the giant linear plasmid SCP1 in various Streptomyces coelicolor strains. J Gen Microbiol 139, 1261–1269.[CrossRef]
    [Google Scholar]
  15. Kinashi, H., Mori, E., Hatani, A. & Nimi, O. ( 1994; ). Isolation and characterization of large linear plasmids from lankacidin-producing Streptomyces species. J Antibiot 47, 1447–1455.[CrossRef]
    [Google Scholar]
  16. Kinashi, H., Fujii, S., Hatani, A., Kurokawa, T. & Shinkawa, H. ( 1998; ). Physical mapping of the linear plasmid pSLA2-L and localization of the eryAI and actI homologs. Biosci Biotechnol Biochem 62, 1892–1897.[CrossRef]
    [Google Scholar]
  17. Kirby, R. & Hopwood, D. A. ( 1977; ). Genetic determination of methylenomycin synthesis by the SCP1 plasmid of Streptomyces coelicolor A3(2). J Gen Microbiol 98, 239–252.[CrossRef]
    [Google Scholar]
  18. Levene, S. D. & Zimm, B. H. ( 1987; ). Separations of open-circular DNA using pulsed-field electrophoresis. Proc Natl Acad Sci U S A 84, 4054–4057.[CrossRef]
    [Google Scholar]
  19. Lezhava, A., Mizukami, T., Kajitani, T., Kameoka, D., Redenbach, M., Shinkawa, H., Nimi, O. & Kinashi, H. ( 1995; ). Physical map of the linear chromosome of Streptomyces griseus. J Bacteriol 177, 6492–6498.
    [Google Scholar]
  20. Lydiate, D. J., Ikeda, H. & Hopwood, D. A. ( 1986; ). A 2·6 kb DNA sequence of Streptomyces coelicolor A3(2) which functions as a transposable element. Mol Gen Genet 203, 79–88.[CrossRef]
    [Google Scholar]
  21. Mochizuki, S., Hiratsu, K., Suwa, M., Ishii, T., Sugino, F., Yamada, K. & Kinashi, H. ( 2003; ). The large linear plasmid pSLA2-L of Streptomyces rochei has an unusually condensed gene organisation for secondary metabolism. Mol Microbiol (in press
    [Google Scholar]
  22. Okanishi, M., Manome, T. & Umezawa, H. ( 1980; ). Isolation and characterization of plasmid DNAs in actinomycetes. J Antibiot 33, 88–91.[CrossRef]
    [Google Scholar]
  23. Pandza, S., Pfalzer, G., Cullum, J. & Hranueli, D. ( 1997; ). Physical mapping shows that the unstable oxytetracycline gene cluster of Streptomyces rimosus lies close to one end of the linear chromosome. Microbiology 143, 1493–1501.[CrossRef]
    [Google Scholar]
  24. Pandza, S., Biukovic, G., Paravic, A., Dadbin, A., Cullum, J. & Hranueli, D. ( 1998; ). Recombination between the linear plasmid pPZG101 and the linear chromosome of Streptomyces rimosus can lead to exchange of ends. Mol Microbiol 28, 1165–1176.[CrossRef]
    [Google Scholar]
  25. Redenbach, M., Ikeda, K., Yamasaki, M. & Kinashi, H. ( 1998; ). Cloning and physical mapping of the EcoRI fragments of the giant linear plasmid SCP1. J Bacteriol 180, 2796–2799.
    [Google Scholar]
  26. Smokvina, T. & Hopwood, D. A. ( 1993; ). Analysis of secondary integration sites for IS117 in Streptomyces lividans and their role in the generation of chromosomal deletions. Mol Gen Genet 239, 90–96.
    [Google Scholar]
  27. Spatz, K., Kohn, H. & Redenbach, M. ( 2002; ). Characterization of the Streptomyces violaceoruber SANK95570 plasmids pSV1 and pSV2. FEMS Microbiol Lett 213, 87–92.[CrossRef]
    [Google Scholar]
  28. Suwa, M., Sugino, H., Sasaoka, A., Mori, E., Fujii, S., Shinkawa, H., Nimi, O. & Kinashi, H. ( 2000; ). Identification of two polyketide synthase gene clusters on the linear plasmid pSLA2-L in Streptomyces rochei. Gene 246, 123–131.[CrossRef]
    [Google Scholar]
  29. Vivian, A. & Hopwood, D. A. ( 1970; ). Genetic control of fertility in Streptomyces coelicolor A3(2): the IF fertility type. J Gen Microbiol 64, 101–117.[CrossRef]
    [Google Scholar]
  30. Vivian, A. & Hopwood, D. A. ( 1973; ). Genetic control of fertility in Streptomyces coelicolor A3(2): new kinds of donor strains. J Gen Microbiol 76, 147–162.[CrossRef]
    [Google Scholar]
  31. Yamasaki, M., Redenbach, M. & Kinashi, H. ( 2001; ). Integrated structures of the linear plasmid SCP1 in two bidirectional donor strains of Streptomyces coelicolor A3(2). Mol Gen Genet 264, 634–642.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.26102-0
Loading
/content/journal/micro/10.1099/mic.0.26102-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error