1887

Abstract

Fluorescent pseudomonads secrete yellow-green siderophores named pyoverdines or pseudobactins. These comprise a dihydroxyquinoline derivative joined to a type-specific peptide and, usually, a carboxylic acid or amide. In strain PAO1, six genes that encode proteins required for pyoverdine synthesis ( genes) have been identified previously. Expression of all of these genes requires an alternative sigma factor PvdS. The purpose of this research was to identify other genes that are required for pyoverdine synthesis in PAO1. Fourteen candidate genes were identified from the PAO1 genome sequence on the basis of their location in the genome, the functions of homologues in other bacteria, and whether their expression was likely to be PvdS-dependent. The candidate genes were mutated and the effects of the mutations on pyoverdine production were determined. Eight new genes were identified. The presence of homologues of genes in other strains of was determined by Southern blotting and in other fluorescent pseudomonads by interrogation of genome sequences. Five genes were restricted to strains of that make the same pyoverdine as strain PAO1, suggesting that they direct synthesis of the type-specific peptide. The remaining genes were present in all strains of that were examined and homologues were present in other species. These genes are likely to direct synthesis of the dihydroxyquinoline moiety and the attached carboxylic acid/amide group. It is likely that most if not all of the genes required for pyoverdine synthesis in PAO1 have now been identified and this will form the basis for a biochemical description of the pathway of pyoverdine synthesis.

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2003-04-01
2020-07-12
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