1887

Abstract

A previous investigation using the Fur titration assay system showed that possesses a gene encoding a protein homologous to IutA, the outer-membrane receptor for ferric aerobactin in . In this study, a 5·6 kb DNA region from the WP1 genome was cloned and two entire genes, and homologues, were identified which are absent from genomic sequences. The IutA and AlcD proteins share 43 % identity with the IutA protein and 24 % identity with the AlcD protein of unknown function, respectively. Primer extension analysis revealed that the gene is transcribed in response to low-iron availability from a putative promoter overlapped with a sequence resembling a consensus Fur-binding sequence. In agreement with the above finding, effectively utilized exogenously supplied aerobactin for growth under iron-limiting conditions. Moreover, insertional inactivation of impaired growth in the presence of aerobactin and incapacitated the outer-membrane fraction from iron-deficient cells for binding Fe-labelled aerobactin. These results indicate that the homologue encodes an outer-membrane protein which functions as the receptor for ferric aerobactin. Southern blot analysis revealed that the homologues are widely distributed in clinical and environmental isolates of . However, additional genes required for ferric aerobactin transport across the inner membrane remain to be clarified.

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2003-05-01
2020-08-12
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