1887

Abstract

infects the human gastric mucosa and elicits an aggressive inflammatory response. Despite the severity of the inflammatory response, the bacterium is able to persist and cause a chronic infection. It is believed that antioxidant defence mechanisms enable this organism to persist. Wild-type strain SS1, and KatA- and KapA-deficient mutants, were used to infect C57/BL6 mice to test this hypothesis. Neither KatA nor KapA was essential for the initial colonization of SS1 in the murine model of infection. The wild-type SS1 colonized the gastric mucosa at significantly higher levels than both mutants throughout the 24-week experiment. Neither KatA- nor KapA-deficient mutants were able to maintain consistent ongoing colonization for the 24-week period, indicating the necessity of both KapA and KatA in sustaining a long-term infection. At 24 weeks, 5/10 mice inoculated with the KatA mutant and 2/10 mice inoculated with the KapA mutant were colonized, compared with 10/10 of the mice inoculated with the wild-type SS1. An increase in the severity of inflammation in the wild-type-inoculated mice appeared to correlate with the decline in colonization of animals inoculated with the mutants, suggesting that increased oxidative stress militated against continued infection by the mutants. These data indicate that KapA may be of equal or greater importance than KatA in terms of sustained infection on inflamed gastric mucosae.

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2003-03-01
2020-08-15
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References

  1. Alm R, Ling L, Moir D.. 20 other authors 1999; Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori . Nature397:176–180
    [Google Scholar]
  2. Beers R., Sizer I. 1952; A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem195:133–140
    [Google Scholar]
  3. Cadenas E. 1989; Biochemistry of oxygen toxicity. Annu Rev Biochem58:79–110
    [Google Scholar]
  4. Crabtree J. 1996; Immune and inflammatory responses to Helicobacter pylori infection. Scand J Gastroenterol31:3–10
    [Google Scholar]
  5. Dixon M. F. 2001; Patterns of inflammation linked to ulcer disease. Best Pract Res Clin Gastroenterol14:27–40
    [Google Scholar]
  6. Enno A, O'Rourke J, Braye S, Howlett R., Lee A. 1998; Antigen-dependent progression of mucosa-associated lymphoid tissue (MALT)-type lymphoma in the stomach. Effects of antimicrobial therapy on gastric MALT lymphoma in mice. Am J Pathol152:1625–1632
    [Google Scholar]
  7. Forman D, Newell D. G, Fullerton F, Yarnell J. W, Stacy A. R, Wald N., Sitas F. 1991; Association between infection with Helicobacter pylori and risk of gastric cancer: evidence from a prospective investigation. Br Med J302:1302–1305
    [Google Scholar]
  8. Frommer D, Carrick J, Lee A., Hazell S. 1988; Acute presentation of Campylobacter pylori gastritis. Am J Gastroenterol83:1168–1171
    [Google Scholar]
  9. Graham D. 1989; Campylobacter pylori and peptic ulcer disease. Gastroenterology96:615–625
    [Google Scholar]
  10. Harris A, Hazell S., Netting A. 2000; Use of Digoxigenin-labelled ampicillin in the identification of penicillin binding proteins in Helicobacter pylori. J Antimicrob Chemother45:591–598
    [Google Scholar]
  11. Harris A. G, Hinds F. E, Beckhouse A. G, Kolesniow T., Hazell S. L. 2002; Resistance to hydrogen peroxide in Helicobacter pylori : role of catalase (KatA) and Fur, and functional analysis of a novel gene product designated ‘KatA-associated protein’, KapA (HP0874). Microbiology 148:3813–3825
    [Google Scholar]
  12. Hazell S, Hennessy W, Borody T, Carrick J, Ralston M, Brady L., Lee A. 1987; Campylobacter pyloridis gastritis. II.: Distribution of bacteria and associated inflammation in the gastroduodenal environment. Am J Gastroenterol82:297–300
    [Google Scholar]
  13. Hazell S, Evans D., Graham D. 1991a; Helicobacter pylori catalase. J Gen Microbiol137:57–61
    [Google Scholar]
  14. Hazell S. L, Evans D. J. Jr, Evans D. G., Graham D. Y. 1991b; Campylobacter pylori catalase: a putative virulence factor. In Campylobacter V (Proceedings of the Vth International Workshop on Campylobacter Infection, Mexico City) pp 367–368 Edited by Ruiz-Palacios G. M., Calva E., Ruiz-Palacios B. R..
    [Google Scholar]
  15. Hazell S. L, Harris A. G., Trend M. A. (eds) 2001; Evasion and avoidance of the toxic effects of oxygen. In Helicobacter pylori: Physiology and Genetics pp 167–175 Edited by Mobley H. L. T., Mendz G. L., Hazell S. L.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  16. Howell M. L, Alsabbagh E, Ma J. F.. 10 other authors 2000; AnkB, a periplasmic ankyrin-like protein in Pseudomonas aeruginosa , is required for optimal catalase B (KatB) activity and resistance to hydrogen peroxide. J Bacteriol182:4545–4556
    [Google Scholar]
  17. Lee A. 2000; Animal models of gastroduodenal ulcer disease. Best Pract Res Clin Gastroenterol14:75–96
    [Google Scholar]
  18. Lee A, O'Rourke J, De Ungria M. C, Robertson B, Daskalopoulos G., Dixon M. F. 1997; A standardized mouse model of Helicobacter pylori infection: introducing the Sydney strain. Gastroenterology112:1386–1397 [an erratum appears in Gastroenterology 113, 732]
    [Google Scholar]
  19. Manos J, Kolesnikow T., Hazell S. L. 1997; An investigation of the molecular basis of the spontaneous occurrence of a catalase negative phenotype in Helicobacter pylori . Helicobacter3:28–38
    [Google Scholar]
  20. McColm A. A, Bagshaw J, O'Mally C., McLaren A. 1995; Development of a mouse model of gastric colonization with Helicobacter pylori . Gut37:A50
    [Google Scholar]
  21. Odenbreit S, Wieland B., Haas R. 1996; Cloning and genetic characterisation of Helicobacter pylori catalase and construction of a catalase deficient mutant. J Bacteriol178:6960–6967
    [Google Scholar]
  22. Parsonnet J, Friedman G, Vandersteen D, Chang Y, Vogelman J, Orenteich N., Sibley R. 1991; Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med325:1127–1131
    [Google Scholar]
  23. Radcliff F. J, Hazell S. L, Kolesnikow T, Doidge C., Lee A. 1997; Catalase, a novel antigen for Helicobacter pylori vaccination. Infect Immun65:4668–4674
    [Google Scholar]
  24. Ramarao N, Gray-Owen S. D., Meyer T. F. 2000; Helicobacter pylori induces but survives the extracellular release of oxygen radicals from professional phagocytes using its catalase activity. Mol Microbiol36:103–113
    [Google Scholar]
  25. Seyler R. W, Olson J. W., Maier R. J. 2001; Superoxide-dismutase deficient mutants of Helicobacter pylori are hypersensitive to oxidative stress and defective in host colonization. Infect Immun69:4034–4040
    [Google Scholar]
  26. Skirrow M. 1977; Campylobacter enteritis: a “new” disease. Br Med J2:9–11
    [Google Scholar]
  27. Sutton P, Kolesniow T, Danon S, Wilson J., Lee A. 2000; Dominant nonresponsiveness to Helicobacter pylori infection is associated with production of interleukin-10 but not gamma interferon. Infect Immun68:4802–4804
    [Google Scholar]
  28. Tomb J, White O, Kerlavage A.. 39 other authors 1997; The complete genome sequence of the gastric pathogen Helicobacter pylori . Nature388:539–547
    [Google Scholar]
  29. Uemura N, Okamoto S, Yamamoto S, Matsumara N, Yamaguchi S, Yamakido M, Taniyama K, Sasaki N., Schlemper R. J. 2001; Helicobacter pylori infection and the development of gastric cancer. N Engl J Med345:784–789
    [Google Scholar]
  30. Westblom T, Phadnis S, Langenberg W, Yoneda K, Madan E., Midkiff B. 1992; Catalase negative mutants of Helicobacter pylori . Eur J Clin Microbiol Infect Dis11:522–526
    [Google Scholar]
  31. Wotherspoon A, Ortitz-Hidalgo C, Falzon M., Isaacson P. 1991; Helicobacter pylori associated gastritis and B-cell gastric lymphoma. Lancet338:1175–1176
    [Google Scholar]
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