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Volume 149, Issue 5

Adhesins encoded by the gingipain genes of are responsible for co-aggregation with Free

Abstract

Co-aggregation among bacterial cells caused by the adherence of one bacterial species to another is a potential colonization mechanism. Several putative aggregation factors for co-aggregation between (.) and (.) were partially purified from vesicles by gel filtration and affinity chromatography. Antisera against the aggregation factors were made. Analysis using these antisera revealed that 18 and 44 kDa proteins might be responsible for vesicle-mediated aggregation of . Using antiserum against the 18 kDa protein, the DNA region encoding it was cloned from genomic DNA. Sequence analysis revealed that the DNA region was located within the and genes, encoding Arg-gingipain (Rgp) and Lys-gingipain (Kgp), respectively, and it encoded non-catalytic adhesin domain regions, namely a C-terminal portion of HGP15, the entire HGP17 sequence and an N-terminal portion of HGP27. A portion of the DNA sequence was also found in the haemagglutinin A () gene. A recombinant glutathione -transferase (GST)–HGP17 fusion protein reacted to antiserum against the 18 kDa protein and cells could adhere to GST–HGP17-conjugated Sepharose 4B beads, indicating that the HGP17 domain protein is responsible for vesicle-mediated aggregation of .

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  • Accepted:
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  • Published Online:
Keyword(s): GST, glutathione S-transferase , Kgp, Lys-gingipain or lysine-specific cysteine proteinase , Por., Porphyromonas , Pre., Prevotella and Rgp, Arg-gingipain or arginine-specific cysteine proteinase
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2003-05-01
2024-04-16
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Adhesins encoded by the gingipain genes of Porphyromonas gingivalis are responsible for co-aggregation with Prevotella intermedia
Microbiology 149, 1257 (2003); https://doi.org/10.1099/mic.0.25997-0
/content/journal/micro/10.1099/mic.0.25997-0
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