1887

Abstract

A regulatory protein, PpaA, involved in photosystem formation in the anoxygenic phototrophic proteobacterium has been identified and characterized . Based on the phenotypes of cells expressing the gene in extra copy and on the phenotype of the null mutant, it was concluded that PpaA activates photopigment production and operon expression under aerobic conditions. This is in contrast to the function of the PpaA homologue from , AerR, which acts as a repressor under aerobic conditions [ Dong, C., Elsen, S., Swem, L. R. & Bauer, C. E. (2002) . 184, 2805–2814]. The expression of the gene increases several-fold in response to a decrease in oxygen tension, suggesting that the PpaA protein is active under conditions of low or no oxygen. However, no discernible phenotype of a null mutant was observed under anaerobic conditions tested thus far. The photosystem gene repressor PpsR mediates repression of gene expression under aerobic conditions. Sequence analysis of PpaA homologues from several anoxygenic phototrophic bacteria revealed a putative corrinoid-binding domain. It is suggested that PpaA binds a corrinoid cofactor and the availability or structure of this cofactor affects PpaA activity.

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2003-02-01
2019-10-23
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