1887

Abstract

A series of shuttle vectors were constructed which contained the replication and maintenance functions of the theta-mode multiresistance plasmid pSK1. The utility of the newly constructed vectors was demonstrated by the successful cloning and expression of several genes that had previously proven difficult to express in . Additional vectors which permit the generation of transcriptional and translational fusions to an reporter gene were also produced and subsequently employed to determine the relative strengths in of a number of promoters. By utilizing the theta-mode replication functions of pSK1, the shuttle vectors described largely avoided the segregational and structural stability problems frequently encountered with Gram-positive rolling-circle-based vectors. In addition, these plasmids represent vectors which are suitable for the analysis of genes in at low copy number.

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2003-03-01
2020-09-21
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