1887

Abstract

The genes for nitrous oxide (NO) reduction, , are clustered on the chromosome of . Promoter assays using transcriptional fusions to revealed that the structural gene for nitrous oxide reductase, , is transcribed with the upstream gene. The gene product is not required for the activity of the promoter. A sequence similar to the consensus FNR-binding motif was found 41·5 bp upstream from the major transcriptional start point of . Mutation of the motif significantly reduced the promoter activity. DNR, an FNR-related transcriptional regulator required for the expression of denitrification genes in , is necessary for the transcription of , indicating that the motif is recognized by DNR. Nitrite (NO−2), nitric oxide (NO) and NO-generating reagents induced promoter activity, but NO did not. The NO−2-induced promoter activity was reduced by mutation of the NO−2 reductase gene. However, a low concentration of NO−2 induced the promoter activity in a NO reductase mutant. These results indicate that NO is the inducer molecule for transcription of the genes.

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2003-01-01
2021-10-16
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