1887

Abstract

The 16S–23S rRNA internal transcribed spacer (ITS) region of several strains and some related dairy streptococci, , and , was analysed by sequence analysis. All the species were easily discriminated on the basis of sequence variations principally located upstream and downstream of the region encompassing the double-stranded processing sites and the tRNA gene. Comparison between tRNA gene sequences highlighted a high level of sequence conservation among the species investigated despite their belonging to separated phylogenetic clusters, i.e. the and rRNA groups. A low but significant degree of variability was detected among the strains, allowing the identification of four different ITS sequences. Similarity analysis of the ITS sequences showed that the species were clustered in two main branches, one containing and strains, and one containing and strains. With the aim of developing a rapid tool for the identification of the dairy streptococci species a multiplex ITS-SSCP analysis of two discrete regions within the ITS locus was carried out.

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2003-03-01
2019-10-19
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