1887

Abstract

In haploid cells, the -specific genes are expressed, whereas in the α haploid and /α diploid cell types their transcription is repressed. It is shown in this report that Itc1p, a component of the ATP-dependent Isw2p–Itc1p chromatin remodelling complex, is required for the repression of -specific genes. It has previously been reported that disruption of the gene leads, in α cells, to an aberrant cell morphology resembling the polarized mating projection of cells responding to pheromone. The activation of the pheromone signalling pathway in mutants of both mating types was examined and found to be constitutively active in α but not in cells. Furthermore, unlike the wild-type, α and / cells secrete -factor and express significant levels of other -specific genes. The results indicate that the inappropriate -factor production in a α context, due to the derepression of the -specific genes, produces an autocrine signalling loop that leads to the aberrant morphology displayed by α cells. It is suggested that the Isw2p–Itc1p complex contributes to maintain the repressive chromatin structure described for the operator present in the promoters of -specific genes.

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2003-02-01
2020-01-28
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