1887

Abstract

possesses two distinct thioredoxin proteins (Trx1 and Trx2) which may play important roles in the ability of this bacterium to survive oxidative stress. Trx1 has previously been shown to be an electron donor for alkyl-hydroperoxide reductase (AhpC), one of three members of the peroxiredoxin family of antioxidant peroxidases present in . In this study, mutants in the and genes encoding Trx1 and Trx2, respectively, and in the and genes, which encode the remaining two members of the peroxiredoxin family, were constructed in order to determine their roles in resistance to damage by reactive oxygen and nitrogen species. Mutation of led to a pronounced increase in sensitivity to oxygen, hydrogen peroxide and the superoxide generator paraquat, as well as to the nitric oxide (NO) releasers sodium nitroprusside (SNP) and -nitrosoglutathione (GSNO), consistent with an role for Trx1 as a reductant for AhpC. A single mutant grew normally in an atmosphere of 2 % (v/v) O but grew very poorly in 10 % (v/v) O. It showed slight increases in killing by hydrogen peroxide, paraquat, SNP and GSNO compared to the wild-type, but was significantly more sensitive to cumene hydroperoxide in disc-diffusion assays. A double mutant was very sensitive to all of the oxidative and nitrosative stresses applied. Comparison of the phenotypes of the and mutants showed that Tpx plays a significant role in peroxide and superoxide resistance in , while the role of Bcp is minimal. No evidence was obtained for a role for either Tpx or Bcp in resistance to the toxic effects of NO. The results show that a functional thioredoxin system is necessary for both oxidative and nitrosative stress resistance in but, surprisingly, is not essential for viability despite the absence of glutathione and a glutaredoxin system in this bacterium.

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2003-01-01
2019-09-22
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