1887

Abstract

is the fourth most common organism responsible for bloodstream infections in many intensive care units, with being the most predominant species isolated in such cases. It has previously been shown that candidal phospholipase B, encoded by the gene, is an important virulence factor for pathogenesis. In this study, the effects of environmental factors (carbohydrate source and pH) and physiological conditions (serum, phospholipids and temperature) on the expression of by cells grown in rich [Sabouraud dextrose broth (SB) or yeast extract/peptone/dextrose] or chemically defined [Lee's, RPMI-1640 or yeast nitrogen base (YNB)] media were investigated. Northern blot analyses revealed that mRNA was expressed in cells grown in rich media at 30 °C but not at 37 °C. However, the protein Plb1p was detected in fungal cells growing at 37 °C in SB, as determined by Western blot analysis, indicating that although the mRNA for this gene was not detected, the actual gene product was present at this temperature. Expression of was detected in cells grown in YNB/glucose at 30 °C but not at 37 °C. However, growth of in YNB/glucose supplemented with serum and phospholipids resulted in expression of at 37 °C also. Additionally, acidic pH induced higher levels of mRNA expression compared to neutral pH, while the morphological form of did not have any influence on the expression of this gene. The studies described here show that the expression of is regulated by nutritional supplementation, environmental factors and the growth phase of the cells, as well as by physiological conditions. The differential expression of in response to environmental factors may be correlated to host-specific components available to during infection.

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2003-01-01
2020-09-24
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