1887

Abstract

The binding of to fibronectin (Fn), a component of the extracellular matrix, is mediated by a 37 kDa outer-membrane protein termed CadF for hesion to ibronectin. The specificity of binding to Fn, via CadF, was demonstrated using antibodies reactive against Fn and CadF. More specifically, the anti-CadF antibody reduced the binding of two clinical isolates to immobilized Fn by greater than 50 %. Furthermore, a wild-type isolate, in contrast to the isogenic CadF mutant, was found to compete with another wild-type isolate for host cell receptors. Given the relationship between the pericellular Fn matrix and the cytoskeleton, the involvement of host cell cytoskeletal components in internalization was also examined. Cytochalasin D and mycalolide B microfilament depolymerizing agents resulted in a significant reduction in invasion. Studies targeting paxillin, a focal adhesion signalling molecule, identified an increased level of tyrosine phosphorylation upon infection of INT 407 cells. Collectively, these data suggest CadF promotes the binding of to Fn, which in turn stimulates a signal transduction pathway involving paxillin.

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2003-01-01
2020-10-24
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