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Volume 149, Issue 1

Molecular analysis of bacteria in periodontitis: evaluation of clone libraries, novel phylotypes and putative pathogens Free

Abstract

Subgingival plaque samples were obtained from 26 subjects with advanced periodontal lesions. Bacterial diversity was analysed by amplification of the 16S rRNA genes with two different primer sets, and subsequent cloning and sequencing. A total of 578 sequences was analysed after the exclusion of chimeras. The authors found 148 phylotypes with the clone library 27f/519r (number of clones =322; coverage, =66 %) and 75 phylotypes with the clone library 515f/1525r (=256; =84 %). Comparative sequence analysis revealed that 70 % of all of the analysed sequences showed a similarity of at least 99 % to sequences deposited in public databases. The classes , , , , , , , , and all classes of the were represented. Sequences that were at least 99 % identical to , and were present in at least one-third of the patients. Libraries generated with the two PCR primer pairs differed significantly in their representation of the families , , , , and . A total of 14 sequences exhibited less than 97 % identity to sequences published previously and were assigned to six different families within the phyla and . Twelve of 20 putative pathogens were recovered, which were recently proposed to be associated with periodontitis.

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  • Published Online:
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2003-01-01
2024-04-20
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Molecular analysis of bacteria in periodontitis: evaluation of clone libraries, novel phylotypes and putative pathogens
Microbiology 149, 67 (2003); https://doi.org/10.1099/mic.0.25791-0
/content/journal/micro/10.1099/mic.0.25791-0
/content/journal/micro/10.1099/mic.0.25791-0
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