1887

Abstract

species bovine group 7, represented by the type strain PG50, is one of six members of the cluster and has been implicated in sporadic and outbreak cases of polyarthritis and mastitis in Australian dairy cattle. This study describes cloning and sequencing a 7·9 kb region of the PG50 chromosome and identification of genes involved in glycerol transport (, and ) that are followed by a putative lipoprotein gene and a genomic locus containing two ORFs encoding putative membrane proteins. Long range PCR using primers spanning and downstream flanking genes, and Southern hybridization analyses using a suite of probes derived from subsp. small colony type (SC) strain Afadé for , and , and the two downstream genes confirmed that these genes were conserved among sp. bovine group 7 isolates and mycoplasmas belonging to the subcluster [ subsp. SC, subsp. large colony type (LC) and subsp. ] but were absent in mycoplasmas belonging to the subcluster ( subsp. and subsp. ). subsp. type strain California kid did not hybridize with the probe for and gave only weak or no hybridization signals with probes derived from the loci downstream of , suggesting that this region has diverged in mycoplasmas belonging to subspecies of . It is shown that PG50, after the addition of a physiological concentration of glycerol to the growth medium, generates HO at levels comparable with strain Afadé, implying that the glycerol transport system is functional in sp. bovine group 7. This suggests that in PG50, as in subsp. SC, glycerol uptake is followed by phosphorylation to glycerol 3-phosphate and then conversion to dihydroxyacetone phosphate, catalysed by -α-glycerophosphate oxidase, resulting in the production of HO. The ability of sp. bovine group 7 to generate significant amounts of hydrogen peroxide may be important in pathogenesis.

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2003-01-01
2019-10-21
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