1887

Microbiology

Volume 154, Issue 11

Metabolic cooperation of sp. strain MTCC 4818 and sp. strain WY in the utilization of butyl benzyl phthalate: effect of a novel co-culture in the degradation of a mixture of phthalates Free

Abstract

Degradation of butyl benzyl phthalate (BBP) by a co-culture of sp. strain MTCC 4818 and sp. strain WY was investigated. In the degradation of BBP by the co-culture, the limitations of the individual species in metabolizing BBP were overcome, leading to the development of a consortium capable of complete utilization of this ester. In the degradation of BBP by the co-culture, the presence of multiple esterases was demonstrated in both species by activity staining of non-denaturing gels, indicating their roles in the degradation process. The esterases were found to be inducible, with unique or broad substrate specificities towards BBP and its monoesters. Moreover, a number of catabolic enzymes other than esterases identified in the metabolism of BBP-degraded intermediates facilitated the co-culture-mediated degradation process. The versatility of the co-culture was further established by the rapid and complete degradation of a mixture of phthalate esters of environmental concern.

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Keyword(s): BBP, butyl benzyl phthalate , D-n-OP, di-n-octyl phthalate , DBP, di-n-butyl phthalate , DCHP, dicyclohexyl phthalate , DEHP, di-(2-ethylhexyl) phthalate , DEP, diethyl phthalate , DIBP, diisobutyl phthalate , DINP, diisononyl phthalate , DMP, dimethyl phthalate , DPP, diphenyl phthalate , MBuP, monobutyl phthalate , MBzP, monobenzyl phthalate , p-NPA, p-nitrophenyl acetate , PAE, phthalic acid ester and TCA, tricarboxylic acid
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2008-11-01
2019-11-13
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Metabolic cooperation of Gordonia sp. strain MTCC 4818 and Arthrobacter sp. strain WY in the utilization of butyl benzyl phthalate: effect of a novel co-culture in the degradation of a mixture of phthalates
Microbiology 154, 3338 (2008); https://doi.org/10.1099/mic.0.2008/021881-0
/content/journal/micro/10.1099/mic.0.2008/021881-0
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