1887

Abstract

is a major food-borne bacterial pathogen, which is capable of causing diarrhoea containing blood and leukocytes. invasion of the intestinal epithelial cells and the release of proinflammatory molecules contribute to the pathophysiology of campylobacteriosis. Given the commensal relationship of with chickens, we hypothesized that invasion of chicken cells and the release of host cell cytokines would be significantly less than with human cells. To test our hypothesis, we examined the interactions of with chicken LMH cells, and performed experiments with chickens. The binding and internalization assays revealed that was significantly less invasive of LMH cells relative to human INT 407 cells, even though the bacteria bound to each host cell species equally. We also assessed interleukin-8 (IL-8) transcript, IL-8 secretion, and the release of chemoattractant molecules from the inoculated cells. Inoculation of LMH cells with stimulated expression of both chicken IL-8 orthologues, and , but at levels significantly less than human IL-8 () expressed from human INT 407 cells inoculated with . Moreover, the supernatant fluids of the inoculated LMH cells resulted in little heterophil migration. , were observed bound to the cells lining the glandular crypts, but overt signs of cell invasion or pathology were not observed. These results indicate that cytokine expression in chicken LMH cells in response to is distinct from that of .

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2008-12-01
2020-08-13
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