1887

Abstract

Adherence to epithelial cells by specific adhesins is a characteristic of Shiga toxin-producing (STEC) strains. The -encoded protein intimin is the main adhesin implicated in intestinal colonization . We recently showed that STEC strains isolated in Chile displayed a wide variety of adhesins; here we demonstrate that some of these STEC strains are -negative and still adhere to epithelial cells at a level 100-fold higher than enterohaemorrhagic (EHEC) O157 : H7 prototype strain EDL933. This phenotype is associated with the presence of adherence factors different from the intimin protein. Subtractive hybridization between EHEC EDL933 and STEC -negative strain 472-1 was used to identify regions implicated in adhesion. In addition to the gene, we identified 18 specific genes in STEC 472-1, 16 of which had nucleotide identity to ST46 phage genes; the two remaining ones shared identity to a gene encoding a hypothetical protein of uropathogenic . The DNA sequence of the STEC 472-1 region identified five open reading frames with homology to phage genes. We constructed mutant strains in the gene and the region to study the participation of these genes in the adherence to epithelial cells and our results demonstrated that STECΔ and STECΔ mutants displayed a 10-fold decrease in adherence as compared to the STEC 472-1 wild-type strain. Overall, our results suggest that STEC strain 472-1 adheres to epithelial cells in an -independent matter and that and participate in this adhesion process.

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2008-12-01
2019-10-23
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DNA sequences of the clones obtained by subtractive hybridization, corresponding to the P4 phage genes found in STEC strain 472-1 [ PDF] (14 kb) Plasmids used in this study [ PDF] (57 kb) Adherence of EHEC O157:H7 strain EDL933 (A) and STEC strain 472-1 (B) to Hep-2 cells examined by transmission electron microscopy [ PDF] (34 kb)

PDF

DNA sequences of the clones obtained by subtractive hybridization, corresponding to the P4 phage genes found in STEC strain 472-1 [ PDF] (14 kb) Plasmids used in this study [ PDF] (57 kb) Adherence of EHEC O157:H7 strain EDL933 (A) and STEC strain 472-1 (B) to Hep-2 cells examined by transmission electron microscopy [ PDF] (34 kb)

PDF

DNA sequences of the clones obtained by subtractive hybridization, corresponding to the P4 phage genes found in STEC strain 472-1 [ PDF] (14 kb) Plasmids used in this study [ PDF] (57 kb) Adherence of EHEC O157:H7 strain EDL933 (A) and STEC strain 472-1 (B) to Hep-2 cells examined by transmission electron microscopy [ PDF] (34 kb)

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