@article{mbs:/content/journal/micro/10.1099/mic.0.2008/020891-0, author = "Hendrickx, Antoni P. A. and Bonten, Marc J. M. and van Luit-Asbroek, Miranda and Schapendonk, Claudia M. E. and Kragten, Angela H. M. and Willems, Rob J. L.", title = "Expression of two distinct types of pili by a hospital-acquired Enterococcus faecium isolate", journal= "Microbiology", year = "2008", volume = "154", number = "10", pages = "3212-3223", doi = "https://doi.org/10.1099/mic.0.2008/020891-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.2008/020891-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "CWS, cell wall sorting signal", keywords = "TEM, transmission electron microscopy", keywords = "PGC, pilin gene cluster", abstract = "Surface filamentous structures designated pili, and implicated in virulence, have been found on the surfaces of several Gram-positive pathogens. This work describes the conditional expression of two phenotypically distinct pilus-like structures, designated PilA and PilB, on the surface of a hospital-adapted Enterococcus faecium bloodstream isolate. E. faecium is an emerging Gram-positive opportunistic pathogen that can cause severe disease, particularly in immunocompromised patients. Expression of PilA- and PilB-type pili was analysed during different phases of growth in broth culture. During growth, PilA and PilB pilin subunits were expressed around the cross-wall in early-exponential-phase cells. Polymerization and migration of short PilB-type pili towards the poles occurred in cells from the exponential phase and long polymerized pili were expressed at the poles of cells grown to stationary phase. In contrast, PilA-type pili were not expressed in broth culture, but only when cells were grown on solid media. Furthermore, surface expression of the PilA- and PilB-type pili was regulated in a temperature-dependent manner, as polymerization of two distinct types of pili at the surface only occurred when cells were grown at 37 °C; no pili were observed on cells grown at 21 °C. Hospital-aquired E. faecium isolates were specifically enriched in pilin gene clusters, suggesting that conditional expression of pili may contribute to E. faecium pathogenesis.", }