RT Journal Article SR Electronic(1) A1 Dienst, Dennis A1 Dühring, Ulf A1 Mollenkopf, Hans-Joachim A1 Vogel, Jörg A1 Golecki, Jochen A1 Hess, Wolfgang R. A1 Wilde, AnnegretYR 2008 T1 The cyanobacterial homologue of the RNA chaperone Hfq is essential for motility of Synechocystis sp. PCC 6803 JF Microbiology, VO 154 IS 10 SP 3134 OP 3143 DO https://doi.org/10.1099/mic.0.2008/020222-0 PB Microbiology Society, SN 1465-2080, AB The ssr3341 locus was previously suggested to encode an orthologue of the RNA chaperone Hfq in the cyanobacterium Synechocystis sp. strain PCC 6803. Insertional inactivation of this gene resulted in a mutant that was not naturally transformable and exhibited a non-phototactic phenotype compared with the wild-type. The loss of motility was complemented by reintroduction of the wild-type gene, correlated with the re-establishment of type IV pili on the cell surface. Microarray analyses revealed a small set of genes with drastically reduced transcript levels in the knockout mutant compared with the wild-type cells. Among the most strongly affected genes, slr1667, slr1668, slr2015, slr2016 and slr2018 stood out, as they belong to two operons that had previously been shown to be involved in motility, controlled by the cAMP receptor protein SYCRP1. This suggests a link between cAMP signalling, motility and possibly the involvement of RNA-based regulation. This is believed to be the first report demonstrating a functional role of an Hfq orthologue in cyanobacteria, establishing a new factor in the control of motility., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.2008/020222-0