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Volume 154, Issue 11

Optical mapping and 454 sequencing of O157 : H7 isolates linked to the US 2006 spinach-associated outbreak Free

Abstract

Optical maps for five representative clinical, food-borne and bovine-derived isolates from the 2006 O157 : H7 outbreak linked to fresh spinach in the United States showed a common set of 14 distinct chromosomal markers that define the outbreak strain. Partial 454 DNA sequencing was used to characterize the optically mapped chromosomal markers. The markers included insertions, deletions, substitutions and a simple single nucleotide polymorphism creating a HI site. The Shiga toxin gene profile of the spinach-associated outbreak isolates ( ) correlated with prophage insertions different from those in the prototypical EDL933 and Sakai reference strains ( ). The prophage occupying the chromosomal position in the spinach-associated outbreak isolates was similar to the EDL933 cryptic prophage V, but it lacked the gene. In EDL933, the genes are within prophage BP933-W at the chromosomal locus; this locus was unoccupied in the spinach outbreak isolates. Instead, the genes were found within a chimeric BP933-W-like prophage with a different integrase, inserted at the locus in the outbreak isolates. An extra set of Shiga toxin genes, , was found in the outbreak isolates within a prophage integrated at the locus. The optical maps of two additional clinical isolates from the outbreak showed a single, different prophage variation in each, suggesting that changes occurred in the source strain during the course of this widespread, multi-state outbreak.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): RFLP, restriction fragment length polymorphism and SNP, single-nucleotide polymorphism
SGM
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2008-11-01
2024-04-19
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Optical mapping and 454 sequencing of Escherichia coli O157 : H7 isolates linked to the US 2006 spinach-associated outbreak
Microbiology 154, 3518 (2008); https://doi.org/10.1099/mic.0.2008/019026-0
/content/journal/micro/10.1099/mic.0.2008/019026-0
/content/journal/micro/10.1099/mic.0.2008/019026-0
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